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Microelectrode biosensor for real-time measurement of ATP in biological tissue
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UNSPECIFIED (2005) Microelectrode biosensor for real-time measurement of ATP in biological tissue. ANALYTICAL CHEMISTRY, 77 (10). pp. 3267-3273. doi:10.1021/ac048106q ISSN 0003-2700.
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Official URL: http://dx.doi.org/10.1021/ac048106q
Abstract
The purines ATP, ADP, and adenosine are important extracellular signaling agents. Analysis of purinergic signaling has been slowed by lack of direct methods for measurement of purine release in real-time during physiological activity. We have previously reported microelectrode biosensors for adenosine, but similar sensors for ATP have remained elusive. We now describe an ATP biosensor formed by coating a Pt microelectrode with an ultrathin biolayer containing glycerol kinase and glycerol-3-phosphate oxidase. It responds rapidly (10-90% rise time < 10 s) and exhibits a linear response to ATP over the physiologically relevant concentrations of 200 nM-50 mu M and is very sensitive similar to 250 mA(.)M(-1.)cm(-2). By including phosphocreatine kinase in the biolayer, we can optionally amplify the ATP signal and also make the sensor sensitive to external ADP. We have used our sensors to make the first demonstration that ATP is released from spinal networks in vivo during locomotor activity.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QD Chemistry | ||||
Journal or Publication Title: | ANALYTICAL CHEMISTRY | ||||
Publisher: | AMER CHEMICAL SOC | ||||
ISSN: | 0003-2700 | ||||
Official Date: | 15 May 2005 | ||||
Dates: |
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Volume: | 77 | ||||
Number: | 10 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 3267-3273 | ||||
DOI: | 10.1021/ac048106q | ||||
Publication Status: | Published |
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