The Library
Enzyme kinetics informatics : from instrument to browser
Tools
Swainston, Neil, Golebiewski, Martin, Messiha, Hanan L., Malys, Naglis, Kania, Renate, Kengne, Sylvestre, Krebs, Olga, Mir, Saqib, Sauer-Danzwith, Heidrun, Smallbone, Kieran, Weidemann, Andreas, Wittig, Ulrike, Kell, Douglas B., Mendes, Pedro, Müller, Wolfgang, Paton, Norman W. and Rojas, Isabel (2010) Enzyme kinetics informatics : from instrument to browser. FEBS Journal, 277 (18). pp. 3769-3779. doi:10.1111/j.1742-4658.2010.07778.x ISSN 1742-464x.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1111/j.1742-4658.2010.07778.x
Abstract
A limited number of publicly available resources provide access to enzyme kinetic parameters. These have been compiled through manual data mining of published papers, not from the original, raw experimental data from which the parameters were calculated. This is largely due to the lack of software or standards to support the capture, analysis, storage and dissemination of such experimental data. Introduced here is an integrative system to manage experimental enzyme kinetics data from instrument to browser. The approach is based on two interrelated databases: the existing SABIO-RK database, containing kinetic data and corresponding metadata, and the newly introduced experimental raw data repository, MeMo-RK. Both systems are publicly available by web browser and web service interfaces and are configurable to ensure privacy of unpublished data. Users of this system are provided with the ability to view both kinetic parameters and the experimental raw data from which they are calculated, providing increased confidence in the data. A data analysis and submission tool, the kineticswizard, has been developed to allow the experimentalist to perform data collection, analysis and submission to both data resources. The system is designed to be extensible, allowing integration with other manufacturer instruments covering a range of analytical techniques.
Item Type: | Journal Article | ||||||
---|---|---|---|---|---|---|---|
Subjects: | Q Science > QP Physiology | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Library of Congress Subject Headings (LCSH): | Enzyme kinetics -- Data processing, Browsers (Computer programs) | ||||||
Journal or Publication Title: | FEBS Journal | ||||||
Publisher: | Blackwell | ||||||
ISSN: | 1742-464x | ||||||
Official Date: | September 2010 | ||||||
Dates: |
|
||||||
Volume: | 277 | ||||||
Number: | 18 | ||||||
Page Range: | pp. 3769-3779 | ||||||
DOI: | 10.1111/j.1742-4658.2010.07778.x | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Funder: | Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Klaus Tschira Foundation (KTF), Germany. Bundesministerium für Bildung und Forschung (BMBF), Heidelberger Institut für Theoretische Studien [Heidelberg Institute for Theoretical Studies] | ||||||
Grant number: | BB⁄C008219⁄1 (EPSRC, BBSRC) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |