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Insufficient histone-3 lysine-9 deacetylation in human oocytes matured in vitro is associated with aberrant meiosis
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Huang, Jia, Li, Tao, Ding, Chen-Hui, Brosens, Jan J., Zhou, Can-Quan, Wang, Hui-Hui and Xu, Yan-Wen (2012) Insufficient histone-3 lysine-9 deacetylation in human oocytes matured in vitro is associated with aberrant meiosis. Fertility and Sterility, 97 (1). pp. 178-184. doi:10.1016/j.fertnstert.2011.10.023 ISSN 0015-0282.
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Official URL: http://dx.doi.org/10.1016/j.fertnstert.2011.10.023
Abstract
Objective:
To characterize histone acetylation during meiosis in human oocytes matured in vitro or in vivo.
Design:
Experimental study.
Setting:
University reproductive medical center.
Patient(s):
Patients undergoing routine intracytoplasmic sperm injection (ICSI) cycles.
Intervention(s):
Immature and mature oocytes were collected from patients undergoing intracytoplasmic sperm injection.
Main Outcome Measure(s):
Immunohistochemical assessment of the levels of acetylated lysine-9 of histone-3 (H3K9) and lysine-12 of histone-4 (H4K12) combined with spindle and chromosome configurations in in vitro– and in vivo–matured human oocytes. Transcript levels of histone deacetylases (HDACs) 1 and 2 were measured by single-cell quantitative polymerase chain reaction.
Result(s):
Acetylation of H3K9 and H4K12 decreased during human oocyte maturation. Residual H3K9 acetylation was found in 37.7% of oocytes matured in vitro, compared with 11.8% in oocytes matured in vivo. Abnormal metaphase spindle was more frequent in oocytes with residual histone acetylation than without (51.6% vs. 25.4%, respectively). Treatment with the HDAC inhibitor trichostatin A increased the incidence of an abnormal metaphase but had no adverse effect on maturation efficiency. Furthermore, expression of HDAC1 transcripts was significantly lower in oocytes matured in vitro versus in vivo.
Conclusion(s):
Reduced HDAC1 expression and insufficient histone deacetylation are associated with metaphase defects in human oocytes matured in vitro.
| Item Type: | Journal Article | ||||||
|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history | ||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Reproductive Health ( - until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Ovum, Meiosis, Histones | ||||||
| Journal or Publication Title: | Fertility and Sterility | ||||||
| Publisher: | Elsevier Inc. | ||||||
| ISSN: | 0015-0282 | ||||||
| Official Date: | January 2012 | ||||||
| Dates: |
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| Volume: | 97 | ||||||
| Number: | 1 | ||||||
| Number of Pages: | 7 | ||||||
| Page Range: | pp. 178-184 | ||||||
| DOI: | 10.1016/j.fertnstert.2011.10.023 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||
| Funder: | Guo jia zi ran ke xue ji jin wei yuan hui (China) [National Natural Science Foundation of China] (NSFC) | ||||||
| Grant number: | 2007CB948103 (NSFC), B2011079 (NSFC) |
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