Cheeseman, Liam P., Booth, Daniel G., Hood, Fiona E., Prior, Ian A. and Royle, Stephen J. (2011) Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges. Communicative & Integrative Biology , 4 (4). pp. 409-412. ISSN 1942-0889.
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Abstract
Accurate chromosome segregation during mitosis is achieved by the kinetochore fibers (K-fibers) of the spindle apparatus. These fibers are bundles of microtubules (MTs) connected by non-motor bridges. We recently identified a TACC3/ch-TOG/clathrin complex that constitutes the shortest class of inter-MT bridge in K-fibers. TACC3 anchors the complex to MTs and this is dependent on phosphorylation by Aurora A kinase. Here we show that inhibition of Aurora A kinase using MLN8237 results in (1) loss of clathrin and TACC3 from spindles, (2) destabilization of K-fibers and (3) loss of inter-MT bridges. These results are similar to those in cells depleted of clathrin or TACC3; suggesting that TACC3/ch-TOG/clathrin bridges are the major class of bridge that is regulated by this kinase.
| Item Type: | Journal Article |
|---|---|
| Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
| Library of Congress Subject Headings (LCSH): | Mitosis, Cancer -- Genetic aspects |
| Journal or Publication Title: | Communicative & Integrative Biology |
| Publisher: | Landes Bioscience |
| ISSN: | 1942-0889 |
| Official Date: | 2011 |
| Dates: | Date Event 2011 Published |
| Volume: | 4 |
| Number: | 4 |
| Number of Pages: | 4 |
| Page Range: | pp. 409-412 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Funder: | Cancer Research UK (CRUK) |
| Grant number: | C25425/A8722 (CRUK) |
| URI: | https://wrap.warwick.ac.uk/70756/ |
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