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Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges
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Cheeseman, Liam P., Booth, Daniel G., Hood, Fiona E., Prior, Ian A. and Royle, Stephen J. (2011) Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges. Communicative & Integrative Biology , 4 (4). pp. 409-412. ISSN 1942-0889.
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Official URL: http://www.tandfonline.com/doi/full/10.4161/cib.15...
Abstract
Accurate chromosome segregation during mitosis is achieved by the kinetochore fibers (K-fibers) of the spindle apparatus. These fibers are bundles of microtubules (MTs) connected by non-motor bridges. We recently identified a TACC3/ch-TOG/clathrin complex that constitutes the shortest class of inter-MT bridge in K-fibers. TACC3 anchors the complex to MTs and this is dependent on phosphorylation by Aurora A kinase. Here we show that inhibition of Aurora A kinase using MLN8237 results in (1) loss of clathrin and TACC3 from spindles, (2) destabilization of K-fibers and (3) loss of inter-MT bridges. These results are similar to those in cells depleted of clathrin or TACC3; suggesting that TACC3/ch-TOG/clathrin bridges are the major class of bridge that is regulated by this kinase.
Item Type: | Journal Article | ||||
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Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Mitosis, Cancer -- Genetic aspects | ||||
Journal or Publication Title: | Communicative & Integrative Biology | ||||
Publisher: | Landes Bioscience | ||||
ISSN: | 1942-0889 | ||||
Official Date: | 2011 | ||||
Dates: |
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Volume: | 4 | ||||
Number: | 4 | ||||
Number of Pages: | 4 | ||||
Page Range: | pp. 409-412 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Cancer Research UK (CRUK) | ||||
Grant number: | C25425/A8722 (CRUK) |
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