The Library
Oxidized polyethylene films for orienting polar molecules for linear dichroism spectroscopy
Tools
Razmkhah, Kasra, Chmel, Nikola Paul, Gibson, Matthew I. and Rodger, Alison (2014) Oxidized polyethylene films for orienting polar molecules for linear dichroism spectroscopy. The analyst, 139 (6). pp. 1372-1382. doi:10.1039/c3an02322b ISSN 0003-2654.
|
PDF
WRAP_c3an02322b.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (1357Kb) | Preview |
Official URL: http://dx.doi.org/10.1039/C3AN02322B
Abstract
Stretched polyethylene (PE) films have been used to orient small molecules for decades by depositing solutions on their surface and allowing the solvent to evaporate leaving the analyte absorbed on the polymer film. However, the non-polar hydrophobic nature of PE is an obstacle to aligning polar molecules and biological samples. In this work PE film was treated with oxygen plasma in order to increase surface hydrophilicity. Different treatment conditions were evaluated using contact angle measurement and X-ray photoelectron spectroscopy. Treated PE (PEOX) films are shown to be able to align molecules of different polarities including progesterone, 1-pyrenecarboxaldehyde, 4′,6-diamidino-2-phenylindole (DAPI) and anthracene. The degree of alignment of each molecule was studied by running series of linear dichroism (LD) experiments and the polarizations of electronic transition moments were determined. For the first time optimal conditions (such as stretching factor and concentration of the sample) for stretched film LD were determined. PEOX aligning ability was compared to that of normal PE films. Progesterone showed a slightly better alignment on PEOX than PE. 1-Pyrenecarboxaldehyde oriented differently on the two different films which enabled transition moment assignment for this low symmetry molecule. DAPI (which does not align on PE) aligned well on PEOX and enabled us to obtain better LD data than had previously been collected with polyvinyl alcohol. Anthracene alignment and formation of dimers and higher order structures were studied in much more detail than previously possible, showing a variety of assemblies on PE and PEOX films.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QD Chemistry | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Library of Congress Subject Headings (LCSH): | Polyethylene -- Oxidation, Biofilms, Linear dichroism | ||||||||
Journal or Publication Title: | The analyst | ||||||||
Publisher: | Royal Society of Chemistry | ||||||||
ISSN: | 0003-2654 | ||||||||
Official Date: | January 2014 | ||||||||
Dates: |
|
||||||||
Volume: | 139 | ||||||||
Number: | 6 | ||||||||
Number of Pages: | 11 | ||||||||
Page Range: | pp. 1372-1382 | ||||||||
DOI: | 10.1039/c3an02322b | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 30 December 2015 | ||||||||
Date of first compliant Open Access: | 30 December 2015 | ||||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Birmingham Science City, Advantage West Midlands (AWM), European Regional Development Fund (ERDF) | ||||||||
Grant number: | BB/F011199/1 (BBSRC) | ||||||||
Adapted As: |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year