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Discrimination between lectins with similar specificities by ratiometric profiling of binding to glycosylated surfaces ; a chemical ‘tongue’ approach
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Otten, Lucienne and Gibson, Matthew I. (2015) Discrimination between lectins with similar specificities by ratiometric profiling of binding to glycosylated surfaces ; a chemical ‘tongue’ approach. RSC Advances , 5 (66). pp. 53911-53914. doi:10.1039/c5ra08857g ISSN 2046-2069.
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Official URL: http://dx.doi.org/10.1039/C5RA08857G
Abstract
Carbohydrate–lectin interactions dictate a range of signalling and recognition processes in biological systems. The exploitation of these, particularly for diagnostic applications, is complicated by the inherent promiscuity of lectins along with their low affinity for individual glycans which themselves are challenging to access (bio)synthetically. Inspired by how a ‘tongue’ can discriminate between hundreds of flavours using a minimal set of multiplexed sensors and a training algorithm, here individual lectins are ‘profiled’ based on their unique binding profile (barcode) to a range of monosaccharides. By comparing the relative binding of a panel of 5 lectins to 3 monosaccharide-coated surfaces, it was possible to generate a training algorithm that enables correct identification of lectins, even those with similar glycan preferences. This is demonstrated to be useful for discrimination between the cholera and ricin toxin lectins showing the potential of this minimalist approach for exploiting glycan complexity.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QP Physiology R Medicine > R Medicine (General) |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
| Library of Congress Subject Headings (LCSH): | Lectins, Carbohydrates , Monosaccharides , Biosensors | ||||||||
| Journal or Publication Title: | RSC Advances | ||||||||
| Publisher: | Royal Society of Chemistry | ||||||||
| ISSN: | 2046-2069 | ||||||||
| Official Date: | 12 June 2015 | ||||||||
| Dates: |
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| Volume: | 5 | ||||||||
| Number: | 66 | ||||||||
| Number of Pages: | 4 | ||||||||
| Page Range: | pp. 53911-53914 | ||||||||
| DOI: | 10.1039/c5ra08857g | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 30 December 2015 | ||||||||
| Date of first compliant Open Access: | 30 December 2015 | ||||||||
| Funder: | Birmingham Science City, Advantage West Midlands (AWM), European Regional Development Fund (ERDF), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) |
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