The Library
Glycerol free cryopreservation of red blood cells enabled by ice recrystallization inhibiting polymers
Tools
Tools
Tools
Deller, Robert C., Vatish, Manu, Mitchell, Daniel E. and Gibson, Matthew I. (2015) Glycerol free cryopreservation of red blood cells enabled by ice recrystallization inhibiting polymers. Biomaterials Science and Engineering, 1 (9). pp. 789-794. doi:10.1021/acsbiomaterials.5b00162 ISSN 2373-9878.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1021/acsbiomaterials.5b00162
Abstract
Cryopreservation is fundamental in prolonging the viabilities of cells and tissues of clinical and biotechnological relevance ex vivo. Furthermore, there is an increasing need to address storage at more easily accessible temperatures in the developing world due to limited resources. Here, the cryopreservation of erythrocytes (red blood cells) with storage at -20 °C using hydroxyethyl starch (HES) and the ice recrystallization inhibitor, poly(vinyl alcohol) (PVA) that is a biomimetic of naturally occurring antifreeze (glyco)proteins (AF(G)Ps) is described. This strategy eliminates the need for high concentrations of membrane penetrating solvents such as glycerol or dimethyl sulphoxide (DMSO). The addition of only 0.1-0.5 wt. % PVA to the polymeric cryoprotectant, HES, significantly enhances cell recovery under conditions that promote damage due to ice recrystallization. The comparative ease with which the addition and removal of both HES and PVA can be attained is an additional attractive quality. Coupled with the benefits attained by the ice recrystallization inhibition activity of PVA, this methodology therefore offers a strategy that could aid the storage and distribution of biological materials.
| Item Type: | Journal Article | ||||||
|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history Q Science > QP Physiology |
||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||
| Library of Congress Subject Headings (LCSH): | Cryopreservation of organs, tissues, etc., Ice crystals -- Growth -- Prevention, Antifreeze proteins , Blood, Erythrocytes | ||||||
| Journal or Publication Title: | Biomaterials Science and Engineering | ||||||
| Publisher: | American Chemical Society | ||||||
| ISSN: | 2373-9878 | ||||||
| Official Date: | 14 September 2015 | ||||||
| Dates: |
|
||||||
| Volume: | 1 | ||||||
| Number: | 9 | ||||||
| Number of Pages: | 5 | ||||||
| Page Range: | pp. 789-794 | ||||||
| DOI: | 10.1021/acsbiomaterials.5b00162 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
| Funder: | Engineering and Physical Sciences Research Council (EPSRC), Royal Society (Great Britain), Warwick Ventures, Advantage West Midlands (AWM), European Regional Development Fund (ERDF), University of Warwick | ||||||
| Grant number: | EP/F500378/1 (EPSRC), Noreen Murray Award (University of Warwick) |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
