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Identification of manganese superoxide dismutase from Sphingobacterium sp. T2 as a novel bacterial enzyme for lignin oxidation
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Rashid, Goran M. M., Taylor, Charles R. (Charles Robert), Liu, Yangqingxu, Zhang, Xiaoyang, Rea, Dean, Fülöp, Vilmos and Bugg, Tim (2015) Identification of manganese superoxide dismutase from Sphingobacterium sp. T2 as a novel bacterial enzyme for lignin oxidation. ACS chemical biology, 10 (10). pp. 2286-2294. doi:10.1021/acschembio.5b00298 ISSN 1554-8929.
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Official URL: http://dx.doi.org/10.1021/acschembio.5b00298
Abstract
The valorisation of aromatic heteropolymer lignin is an important unsolved problem in the development of a biomass-based biorefinery, for which novel high-activity biocatalysts are needed. Sequencing of the genomic DNA of lignin-degrading bacterial strain Sphingobacterium sp. T2 revealed no matches to known lignin-degrading genes. Proteomic matches for two manganese superoxide dismutase proteins were found in partially purified extracellular fractions. Recombinant MnSOD1 and MnSOD2 were both found to show high activity for oxidation of Organosolv and Kraft lignin, and lignin model compounds, generating multiple oxidation products. Structure determination revealed that the products result from aryl-Cα and Cα-Cβ bond oxidative cleavage and O-demethylation. The crystal structure of MnSOD1 was determined to 1.35 Å resolution, revealing a typical MnSOD homodimer harbouring a 5-coordinate trigonal bipyramidal Mn(II) centre ligated by three His, one Asp and a water/hydroxide in each active site. We propose that the lignin oxidation reactivity of these enzymes is due to the production of hydroxyl radical, a highly reactive oxidant. This is the first demonstration that MnSOD is a microbial lignin-oxidising enzyme.
Item Type: | Journal Article | ||||||
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Subjects: | Q Science > QK Botany | ||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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Library of Congress Subject Headings (LCSH): | Lignin -- Biodegradation | ||||||
Journal or Publication Title: | ACS chemical biology | ||||||
Publisher: | American Chemical Society | ||||||
ISSN: | 1554-8929 | ||||||
Official Date: | 21 July 2015 | ||||||
Dates: |
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Volume: | 10 | ||||||
Number: | 10 | ||||||
Page Range: | pp. 2286-2294 | ||||||
DOI: | 10.1021/acschembio.5b00298 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||
Date of first compliant deposit: | 30 December 2015 | ||||||
Date of first compliant Open Access: | 28 July 2016 | ||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Kurdistān (Iraq). Ḧikûmetî Herêmî Kurdistan-ʻÊraq [Kurdistan Regional Government], Tuck Foundation | ||||||
Grant number: | BB/M003523/1 (BBSRC) |
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