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Biocatalytic conversion of lignin to aromatic dicarboxylic acids in Rhodococcus jostii RHA1 by re-routing aromatic degradation pathways
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Mycroft, Zoe, Gomis, Maria, Mine, Paul, Law, Paul and Bugg, Tim (2015) Biocatalytic conversion of lignin to aromatic dicarboxylic acids in Rhodococcus jostii RHA1 by re-routing aromatic degradation pathways. Green chemistry, 17 (11). pp. 4974-4979. doi:10.1039/c5gc01347j ISSN 1463-9262.
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Official URL: http://dx.doi.org/10.1039/C5GC01347J
Abstract
The heteropolymer lignin represents an untapped resource for production of renewable aromatic chemicals, if efficient depolymerisation methods can be developed. In this work, the metabolic pathways in Rhodococcus jostii RHA1 for degradation of aromatic lignin breakdown products are re-routed, in order to generate an aromatic dicarboxylic acid product that could be used for bioplastic synthesis. Protocatechuic acid is normally metabolised via ortho-cleavage to the β-keto-adipate pathway. Insertion of recombinant genes for protocatechuate 4,5-dioxygenase or protocatechuate 2,3-dioxygenase into R. jostii RHA1, followed by ammonia cyclisation of the extradiol cleavage products, generates pyridine 2,4-dicarboxylic acid or pyridine 2,5-dicarboxylic acid bioproducts in yields of 80–125 mg L−1 when grown on minimal media containing 1% wheat straw lignocellulose.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QD Chemistry | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||||
Library of Congress Subject Headings (LCSH): | Lignin -- Biodegradation, Polymerization | ||||||||||
Journal or Publication Title: | Green chemistry | ||||||||||
Publisher: | Royal Society of Chemistry | ||||||||||
ISSN: | 1463-9262 | ||||||||||
Official Date: | 1 November 2015 | ||||||||||
Dates: |
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Volume: | 17 | ||||||||||
Number: | 11 | ||||||||||
Page Range: | pp. 4974-4979 | ||||||||||
DOI: | 10.1039/c5gc01347j | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Funder: | Technology Strategy Board (Great Britain) | ||||||||||
Grant number: | TP131141 (TSB) |
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