The Library
Role for astroglial α1-adrenoreceptors in gliotransmission and control of synaptic plasticity in the neocortex
Tools
Pankratov, Yuriy and Lalo, Ulyana (2015) Role for astroglial α1-adrenoreceptors in gliotransmission and control of synaptic plasticity in the neocortex. Frontiers in Cellular Neuroscience, 9 . 230. doi:10.3389/fncel.2015.00230 ISSN 1662-5102.
|
PDF
WRAP_fncel-09-00230.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons: Attribution-Noncommercial 4.0. Download (1671Kb) | Preview |
Official URL: http://dx.doi.org/10.3389/fncel.2015.00230
Abstract
Communication between neuronal and glial cells is thought to be very important for many brain functions. Acting via release of gliotransmitters, astrocytes can modulate synaptic strength. The mechanisms underlying gliotransmission remain uncertain with exocytosis being the most intriguing and debated pathway. We demonstrate that astroglial α1-adrenoreceptors are very sensitive to noradrenaline (NA) and make a significant contribution to intracellular Ca2+-signaling in layer 2/3 neocortical astrocytes. We also show that astroglial α1-adrenoreceptors are prone to desensitization upon prolonged exposure to NA. We show that within neocortical slices, α-1adrenoreceptors can activate vesicular release of ATP and D-serine from cortical astrocytes which initiate a burst of ATP receptor-mediated currents in adjacent pyramidal neurons. These purinergic currents can be inhibited by intracellular perfusion of astrocytes with Tetanus Toxin light chain, verifying their origin via astroglial exocytosis. We show that α1 adrenoreceptor-activated release of gliotransmitters is important for the induction of synaptic plasticity in the neocortex:long-term potentiation (LTP) of neocortical excitatory synaptic potentials can be abolished by the selective α1-adrenoreceptor antagonist terazosin. We show that weak sub-threshold theta-burst stimulation (TBS) can induce LTP when astrocytes are additionally activated by 1 μM NA. This facilitation is dependent on the activation of neuronal ATP receptors and is abolished in neocortical slices from dn-SNARE mice which have impaired glial exocytosis. Importantly, facilitation of LTP by NA can be significantly reduced by perfusion of individual astrocytes with Tetanus Toxin. Our results strongly support the physiological importance of astroglial adrenergic signaling and exocytosis of gliotransmitters for modulation of synaptic transmission and plasticity.
Item Type: | Journal Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QH Natural history | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Exocytosis, Astrocytes, Neureplasticity, Neocortex, Neural transmission, Adenosine triphosphate | ||||||||
Journal or Publication Title: | Frontiers in Cellular Neuroscience | ||||||||
Publisher: | Frontiers Research Foundation | ||||||||
ISSN: | 1662-5102 | ||||||||
Official Date: | 16 June 2015 | ||||||||
Dates: |
|
||||||||
Volume: | 9 | ||||||||
Article Number: | 230 | ||||||||
DOI: | 10.3389/fncel.2015.00230 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 30 December 2015 | ||||||||
Date of first compliant Open Access: | 30 December 2015 | ||||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||
Grant number: | BB/K009192/1 (BBSRC) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year