The Library
Characterization of para-nitrophenol degrading bacterial communities in river water using functional markers and stable isotope probing
Tools
Kowalczyk, Agnieszka, Eyice, Özge, Schäfer, Hendrik, Price, Oliver R, Finnegan, Christopher J., van Egmond, Roger A., Shaw, Liz J., Barrett, Glyn and Bending, G. D. (2015) Characterization of para-nitrophenol degrading bacterial communities in river water using functional markers and stable isotope probing. Applied and Environmental Microbiology, 81 (19). pp. 6890-6900. AEM.01794-15. doi:10.1128/AEM.01794-15 ISSN 0099-2240.
|
PDF
WRAP_PNP_manuscript_final_17072015.pdf - Accepted Version - Requires a PDF viewer. Download (1168Kb) | Preview |
Official URL: http://dx.doi.org/10.1128/AEM.01794-15
Abstract
Microbial degradation is a major determinant of pollutant fate in the environment. Para- 23 nitrophenol (PNP) is an EPA listed priority pollutant with wide environmental distribution 24 but little is known about the microorganisms that degrade it in the environment. We studied 25 the diversity of active PNP- degrading bacterial populations in river water using a novel functional marker approach coupled with [13 26 C6]-PNP Stable Isotope Probing (SIP). Culturing 27 together with culture independent Terminal Restriction Fragment Length Polymorphism 28 analysis of 16S rRNA gene amplicons identified Pseudomonas syringae as the major driver 29 of PNP degradation in river water microcosms. This was confirmed by SIP-pyrosequencing
30 of amplified 16S rRNA. Similarly, functional gene analysisshowed that degradation followed 31 the Gram-negative pathway involving pnpA from Pseudomonas spp. However analysis of 32 maleylacetate reductase (mar), an enzyme common to late stages of both Gram negative and 33 Gram positive bacterial PNP degradation pathways, identified a diverse assemblage of 34 bacteria associated with PNP degradation, suggesting that mar had limited use as a specific 35 marker of PNP biodegradation. Both pnpA and mar genes were detected in a PNP-degrading 36 isolate, P. syringae AKHD2 which was isolated from river water. Our results suggest that 37 PNP degrading cultures of Pseudomonas spp. were representative of environmental PNP 38 degrading populations.
Item Type: | Journal Article | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QH Natural history | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
Library of Congress Subject Headings (LCSH): | Chemicals -- Biodegradation -- Research, Water -- Pollution -- Environmental aspects, Microorganisms, Pseudomonas syringae | ||||||||||
Journal or Publication Title: | Applied and Environmental Microbiology | ||||||||||
Publisher: | American Society for Microbiology | ||||||||||
ISSN: | 0099-2240 | ||||||||||
Official Date: | October 2015 | ||||||||||
Dates: |
|
||||||||||
Volume: | 81 | ||||||||||
Number: | 19 | ||||||||||
Page Range: | pp. 6890-6900 | ||||||||||
Article Number: | AEM.01794-15 | ||||||||||
DOI: | 10.1128/AEM.01794-15 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 30 December 2015 | ||||||||||
Date of first compliant Open Access: | 24 May 2016 | ||||||||||
Funder: | Unilever (Firm), Natural Environment Research Council (Great Britain) (NERC) | ||||||||||
Grant number: | NE/J014168/1 |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year