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Population structure of Helicobacter pylori among ethnic groups in Cameroon
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Nell, S., Eibach, D., Montano, V., Nkwesheu, A., Maady, A., Linz, B., Moodley, Y., Achtman, Mark and Suerbaum, S. (2012) Population structure of Helicobacter pylori among ethnic groups in Cameroon. In: 64. Jahrestagung der Deutschen Gesellschaft fur Hygiene und Mikrobiologie (DGHM). Published in: International Journal of Medical Microbiology, 302 p. 116. doi:10.1016/j.ijmm.2012.08.001 ISSN 1438-4221.
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Official URL: http://dx.doi.org/10.1016/j.ijmm.2012.08.001
Abstract
Introduction: The association between the major gastric pathogen Helicobacter pylori and humans dates back at least 100,000 years. Since that time H. pylori evolved in parallel with its human host, accompanying anatomically modern humans ‘out of Africa’ and differentiating into seven known biogeographic populations. Three of these are indigenous to Africa: hpNEAfrica, hpAfrica1, and hpAfrica2. The most divergent H. pylori population, hpAfrica2, is associated with the ancient San hunter-gatherers of southern Africa. This striking parallel between the oldest human and bacterial lineages led us to investigate the prevalence and genetic structure of H. pylori in another ancient hunter-gatherer population, the Cameroonian Baka Pygmies.
Methods: Gastric biopsies were obtained for a total of 178 individuals, 77 of them belonging to the Baka Pygmy population and the remainder to neighboring agricultural Bantu communities. H. pylori was cultured from both antrum and corpus biopsies from each individual. The isolates were analyzed using MLST (Multilocus sequence typing). The population structure of distinct haplotypes was determined by Bayesian clustering using the program STRUCTURE. Phylogenetic analyses were perfomed using ClonalFrame.
Results: The H. pylori prevalence among Baka pygmies (20%) was significantly lower compared to the non-Baka individuals (80%). MLST analysis of all isolates identified 113 unique haplotypes, ten of which were shared by more than one person. Subsequent STRUCTURE analyses assigned these isolates to either hpNEAfrica (67), hpAfrica1 (44), or hpEurope (2) populations. Among infected Baka and non-Baka individuals, the infection rates with hpNEAfrica and hpAfrica1 isolates were similar. In phylogenetic analyses using ClonalFrame we observed an intermediate position of the Cameroonian hpAfrica1 isolates between the already described hspSAfrica and hspWAfrica subpopulations corresponding to the geographic location of Cameroon between South and West Africa. Further STRUCTURE analyses of the hpNEAfrica population revealed a possible subdivision into an East African and a Central African subpopulation.
Discussion: The results suggest that the Baka pygmies, which belong to one of the oldest branches of the human population tree and still live as hunter-gatherers, were initially H. pylori free and acquired H. pylori more recently from their neighboring Bantu communities.
Item Type: | Conference Item (Paper) | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | International Journal of Medical Microbiology | ||||
Publisher: | Elsevier GmbH | ||||
ISSN: | 1438-4221 | ||||
Official Date: | September 2012 | ||||
Dates: |
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Volume: | 302 | ||||
Page Range: | p. 116 | ||||
DOI: | 10.1016/j.ijmm.2012.08.001 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Description: | Annual Meeting of the German Society for Hygiene and Microbiology (DGHM) |
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Conference Paper Type: | Paper | ||||
Title of Event: | 64. Jahrestagung der Deutschen Gesellschaft fur Hygiene und Mikrobiologie (DGHM) | ||||
Type of Event: | Conference |
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