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Exploring the dynamics and mutational landscape of riboregulation with a minimal synthetic circuit in living cells
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Rodrigo, Guillermo, Majer, Eszter, Prakash, Satya, Daros, Jose Antonio, Jaramillo, Alfonso and Poyatos, Juan F. (2015) Exploring the dynamics and mutational landscape of riboregulation with a minimal synthetic circuit in living cells. Biophysical Journal, 109 (5). pp. 1070-1076. doi:10.1016/j.bpj.2015.07.021 ISSN 0006-3495.
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WRAP_0380721-lf-270815-rodrigo-exploring_the_dynamics_and_mutational_landscape_of_riboregulation_with_a_minimal_synthetic_circuit_in_living_cells-2015 (1).pdf - Accepted Version - Requires a PDF viewer. Download (676Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.bpj.2015.07.021
Abstract
Regulation of gene expression triggered by conformational changes in RNA molecules is widely observed in cellular systems. Here, we examine this mode of control by means of a model-based design
and construction of a fully synthetic riboregulatory device. We present a theoretical framework that rests on a simple energy model to predict the dynamic response of such a system. Following an equilibrium description, our framework integrates thermodynamic properties –anticipated with an RNA physicochemical model– with a detailed description of the intermolecular interaction. The theoretical calculations are confirmed with an experimental characterization of the action of the riboregulatory device within living cells. This illustrates, more broadly, the predictability of genetic robustness on synthetic systems, and the faculty to engineer gene expression programs from a minimal set of first principles.
| Item Type: | Journal Article | ||||||||
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| Alternative Title: | |||||||||
| Subjects: | Q Science > QH Natural history > QH426 Genetics | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| Library of Congress Subject Headings (LCSH): | Cells, RNA, Nucleic acids | ||||||||
| Journal or Publication Title: | Biophysical Journal | ||||||||
| Publisher: | Cell Press | ||||||||
| ISSN: | 0006-3495 | ||||||||
| Official Date: | 1 September 2015 | ||||||||
| Dates: |
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| Volume: | 109 | ||||||||
| Number: | 5 | ||||||||
| Page Range: | pp. 1070-1076 | ||||||||
| DOI: | 10.1016/j.bpj.2015.07.021 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Date of first compliant deposit: | 31 December 2015 | ||||||||
| Date of first compliant Open Access: | 1 September 2016 |
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