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Enzymatically-triggered, isothermally responsive polymers : re-programming poly(oligoethylene glycols) to respond to phosphatase
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Phillips, Daniel J., Wilde, Marleen, Greco, Francesca and Gibson, Matthew I. (2015) Enzymatically-triggered, isothermally responsive polymers : re-programming poly(oligoethylene glycols) to respond to phosphatase. Biomacromolecules, 16 (10). pp. 3256-3264. doi:10.1021/acs.biomac.5b00929 ISSN 1525-7797.
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WRAP_0970074-ch-010915-article_revised.pdf - Accepted Version - Requires a PDF viewer. Download (944Kb) | Preview |
Official URL: http://dx.doi.org/10.1021/acs.biomac.5b00929
Abstract
Polymers which can respond to externally applied stimuli have found much application in the biomedical field due to their (reversible) coil-globule transitions. Polymers displaying a lower critical solution temperature are the most commonly used, but for blood-borne (i.e. soluble) biomedical applications the application of heat is not always possible, nor practical. Here we report the design and synthesis of poly(oligoethylene glycol methacrylate)-based polymers whose cloud points are easily varied by alkaline phosphatase-mediated dephosphorylation. By fine-tuning the density of phosphate groups on the backbone, it was possible to induce an isothermal transition: A change in solubility triggered by removal of a small number of phosphate esters from the side chains activating the LCST-type response. As there was no temperature change involved, this serves as a model of a cell-instructed polymer response. Finally, it was found that both polymers were non cytotoxic against MCF-7 cells (at 1 mg.mL-1), which confirms promise for biomedical applications.
Item Type: | Journal Article | ||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Library of Congress Subject Headings (LCSH): | Biopolymers, Polymers in medicine | ||||||||
Journal or Publication Title: | Biomacromolecules | ||||||||
Publisher: | American Chemical Society | ||||||||
ISSN: | 1525-7797 | ||||||||
Official Date: | 28 August 2015 | ||||||||
Dates: |
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Volume: | 16 | ||||||||
Number: | 10 | ||||||||
Page Range: | pp. 3256-3264 | ||||||||
DOI: | 10.1021/acs.biomac.5b00929 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Date of first compliant deposit: | 8 December 2015 | ||||||||
Date of first compliant Open Access: | 28 August 2016 | ||||||||
Funder: | Advantage West Midlands (AWM), European Regional Development Fund (ERDF), University of Warwick, University of Reading |
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