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Reticulomics : protein-protein interaction studies with two plasmodesmata-localised reticulon family proteins identify binding partners enriched at plasmodesmata, ER and the plasma membrane
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Kriechbaumer, Verena, Botchway, Stanley W., Slade, Susan E., Knox, Kirsten, Frigerio, Lorenzo, Oparka, Karl J. and Hawes, Chris (2015) Reticulomics : protein-protein interaction studies with two plasmodesmata-localised reticulon family proteins identify binding partners enriched at plasmodesmata, ER and the plasma membrane. Plant Physiology, 169 (3). pp. 1933-1945. doi:10.1104/pp.15.01153
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9870459-lf-110915-plant_physiol.-2015-kriechbaumer-pp.15.01153.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (2116Kb) |
Official URL: http://dx.doi.org/10.1104/pp.15.01153
Abstract
The ER is a ubiquitous organelle that plays roles in secretory protein production, folding, quality control, and lipid biosynthesis. The cortical ER in plants is pleomorphic and structured as a tubular network capable of morphing into flat cisternae, mainly at three way junctions, and back to tubules. Plant reticulon (RTNLB) proteins tubulate the ER by dimer- and oligomerization, creating localised ER membrane tensions that result in membrane curvature. Some RTNLB ER-shaping proteins are present in the plasmodesmal (PD) proteome (Fernandez-Calvino et al., 2011) and may contribute to the formation of the desmotubule, the axial ER-derived structure that traverses primary PD (Knox et al., 2015). Here we investigate the binding partners of two PD-resident reticulon proteins, RTNLB3 and RTNLB6, that are located in primary PD at cytokinesis (Knox et al., 2015). Co-immunoprecipitation of GFP-tagged RTNLB3 and RTNLB6 followed by mass spectrometry detected a high percentage of known PD-localised proteins as well as plasma-membrane proteins with putative membrane anchoring roles. FRET-FLIM assays revealed a highly significant interaction of the detected PD proteins with the bait RTNLB proteins. Our data suggest that RTNLB proteins, in addition to a role in ER modelling, may play important roles in linking the cortical ER to the plasma membrane.
| Item Type: | Journal Article | ||||||||||
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||
| Journal or Publication Title: | Plant Physiology | ||||||||||
| Publisher: | American Society of Plant Biologists | ||||||||||
| ISSN: | 0032-0889 | ||||||||||
| Official Date: | November 2015 | ||||||||||
| Dates: |
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| Date of first compliant deposit: | 31 December 2015 | ||||||||||
| Volume: | 169 | ||||||||||
| Number: | 3 | ||||||||||
| Page Range: | pp. 1933-1945 | ||||||||||
| DOI: | 10.1104/pp.15.01153 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access |
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