Haj Kassem, Amin A. and Walsh, John A.. (2008) Characterising resistance to Turnip mosaic virus (TuMV) in Turnip (Brassica rapa rapa). Arab Journal of Plant Protection, Vol.26 (No.2). pp. 168-172. ISSN 0255-982X

Preview

PDF WRAP_Walsh_Characterising_resistance_turnip.pdf - Requires a PDF viewer such as GSview, Xpdf or Adobe Acrobat Reader Download (237Kb)

Abstract

A Brassica rapa rapa L. line has been identified with high resistance to seven isolates of Turnip mosaic virus (TuMV) (including UK 1, CHN 5, CZE 1, CDN 1, GBR 6, POL 1 and UK 4) representing the major pathotypes of the virus. Resistant plants showed no symptoms following mechanical inoculation with TuMV and no virus was detected in the plants by ELISA. A cross was made between the rapid-cycling Brassica rapa line R-o-18 (which has been found to be susceptible to all the TuMV isolates) and a plant from the resistant B. rapa rapa line. The small amount of the F1 generation seed available from this cross has been grown and inoculated with the seven TuMV isolates. F1 plants were uniformly resistant to the UK 1 isolate of TuMV, uniformly susceptible to the CHN 5 isolate (only 2 plants inoculated) and segregated for resistance and susceptibility to the other five TuMV isolates. This suggested that the parent B. rapa rapa plant used in the cross was probably homozygous for one, or more dominant resistance genes to the UK 1 isolate of TuMV and heterozygous for one, or more dominant resistance genes to the other TuMV isolates. When self seed (S1) from the parent plant from the resistant line was inoculated with the TuMV isolates GBR 6 and UK 4, the segregation for the former isolate was not significantly different from 3 resistant to 1 susceptible, whereas for the latter isolate, the segregation was 4 resistant to 9 susceptible, suggesting resistance to GBR 6 is controlled by a single dominant gene, whereas resistance to UK 4 is controlled by two or more dominant resistance genes. The putative resistance genes appear to confer hitherto unknown dominant TuMV resistance specificities, and in combination have the exciting potential of providing durable resistance to TuMV.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology S Agriculture > SB Plant culture
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Turnip mosaic virus, Turnips -- Disease and pest resistance, Turnips -- Diseases and pests, Plants -- Virus resistance -- Genetic aspects, Natural immunity
Journal or Publication Title:	Arab Journal of Plant Protection
Publisher:	Arab Society for Plant Protection
ISSN:	0255-982X
Date:	December 2008
Volume:	Vol.26
Number:	No.2
Page Range:	pp. 168-172
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Description:	Item chiefly in English with abstract in English and Arabic.
References:	1. Broadbent, L. 1957. Investigation of virus diseases of brassica crops. Agricultural Research Council Report Series No. 14, Cambridge University Press, Cambridge, UK. 94 pp. 2. Edwardson, J.R. and R.G. Christie. 1991. The Potyvirus Group. University of Florida Monograph 16, Volume 3: 699-712. Gainesville, USA: University of Florida. 3. Green, S.K. and T.C. Deng. 1985. Turnip mosaic virus strains in cruciferous hosts in Taiwan. Plant Disease, 69: 28–31. 4. Hughes, S.L., P.J. Hunter, A.G. Sharpe, M.J. Kearsey, D.J. Lydiate and J.A. Walsh. 2003. Genetic mapping of a novel Turnip mosaic virus resistance gene in Brassica napus. Theoretical and Applied Genetics, 107: 1169–1173 5. Jenner, C.E. and J.A. Walsh. 1996. Pathotypic variation in turnip mosaic virus with special reference to European isolates. Plant Pathology, 45: 848–56. 6. Jenner, C.E., G.J. Keane, J.E. Jones and J.A. Walsh. 1999. Serotypic variation in turnip mosaic virus. Plant Pathology, 48: 101–108. 7. Jenner, C.E., F. Sanchez, S.B. Nettleship, G.D. Foster, F. Ponz and J.A. Walsh. 2000. The cylindrical inclusion gene of turnip mosaic virus encodes a pathogenic determinant to the Brassica resistance gene TuRB01. Molecular Plant-Microbe Interactions, 13: 1102-1108. 8. Jenner, C.E., X. Wang, K. Tomimura, K. Ohshima, F. Ponz and J.A. Walsh. 2003. The dual role of the potyvirus P3 protein of Turnip mosaic virus as a symptom and avirulence determinant in brassicas. Molecular Plant-Microbe Interactio ns, 16: 777-784. 9. Kennedy, J.S., M.F. Day and V.F. Eastop. 1962. A conspectus of aphids as vectors of plant viruses. Commonwealth Institute of Entomology, London. 114 pp. 10. Liu, X.P., W.C. Lu, Y.K. Liu and J.L. Li. 1990. A study on TuMV strain differentiation of cruciferous vegetables from ten provinces in China. Chinese Science Bulletin, 35: 1734–1739. 11. Provvidenti, R. 1980. Evaluation of Chinese cabbage cultivars from Japan and the People’s Republic of China for resistance to turnip mosaic virus and cauliflower mosaic virus. Journal American Society of Horticultural Science, 105: 571–573. 12. Robbins, M.A., H. Witsenboer, R.W. Michelmore, J.F. Laliberte and M.G. Fortin. 1994. Genetic mapping of turnip mosaic virus resistance in Lactuca sativa. Theoretical and Applied Genetics, 89: 583-589. 13. Rusholme, R.L. 2000. The genetic control of resistance to turnip mosaic virus (TuMV) in Brassica. PhD Thesis, University of East Anglia. 112 pp. 14. Rusholme, R.L., E.E. Higgins, J.A. Walsh and D.J. Lydiate. 2007. Genetic control of broad-spectrum resistance to turnip mosaic virus in Brassica rapa (Chinese cabbage). Journal of General Virology, 88: 3177-3186. 15. Shattuck, V.I. 1992. The biology, epidemiology and control of turnip mosaic virus. Plant Breeding Reviews, 14: 199–238. 16. Shukla, D.D., C.W. Ward and A.A. Brunt. 1994. The Potyviridae. Wallingford, UK: CAB International. 516 pp. 17. Stobbs, L.W. and V.I. Shattuck. 1989. Turnip mosaic virus strains in southern Ontario, Canada. Plant Disease, 75: 575–9. 18. Suh S.K., S.K. Green and H.G. Park. 1995. Genetics of resistance to five strains of turnip mosaic virus in Chinese cabbage. Euphytica, 81: 71–77. 19. Tomlinson, J.A. 1987. Epidemiology and control of virus diseases of vegetables. Annals of Applied Biology, 110: 661–81. 20. Walsh, J.A. 1989. Genetic control of immunity to turnip mosaic virus in winter oilseed rape (Brassica napus ssp. oleifera) and the effect of foreign isolates of the virus. Annals of Applied Biology, 115: 89-99. 21. Walsh, J.A. and C.E. Jenner. 2002. Turnip mosaic virus and the quest for durable resistance. Molecular Plant Pathology, 3: 289-300. 22. Walsh, J.A. and J.A. Tomlinson. 1985. Viruses infecting winter oilseed rape (Brassica napus ssp. oleifera). Annals of Applied Biology, 107: 485–95. 23. Walsh, J.A., A.G. Sharpe, C.E. Jenner and D.J. Lydiate. 1999. Characterisation of resistance to turnip mosaic virus in oilseed rape (Brassica napus) and genetic mapping of TuRB01.Theoretical and Applied Genetics, 99: 1149–54. 24. Walsh, J.A., R.L. Rusholme, S.L. Hughes, C.E. Jenner, J.M. Bambridge, D.J. Lydiate and S.K. Green. 2002. Different classes of resistance to turnip mosaic virus in Brassica rapa. European Journal of Plant Pathology, 108: 15–20. 25. Yoon, J.Y., S.K. Green and R.T. Opena. 1993. Inheritance of resistance to turnip mosaic virus in Chinese cabbage. Euphytica, 69: 103–108.
URI:	http://wrap.warwick.ac.uk/id/eprint/726

Request changes to a record

Actions (login required)

View Item