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The role of the Cucumber mosaic virus 2b protein in viral movement and symptom induction

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Lewsey, Mathew, Surette, Monique, Robertson, Fiona C. , Ziebell, Heiko, Choi, Sun Hee , Ryu, Ki Hyun , Canto, Tomas, Palukaitis, Peter, Payne, Tina, Walsh, John A. and Carr, John P.. (2009) The role of the Cucumber mosaic virus 2b protein in viral movement and symptom induction. Molecular Plant - Microbe Interactions, Vol.22 (No.6). pp. 642-654. ISSN 0894-0282

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Official URL: http://dx.doi.org/10.1094/MPMI-22-6-0642

Abstract

The Cucumber mosaic virus (CMV) 2b protein is a counter-defense factor and symptom determinant. Conserved domains in the 2b protein sequence were mutated in the 2b gene of strain Fny-CMV. The effects of these mutations were assessed by infection of Nicotiana tabacum, N. benthamiana, and Arabidopsis thaliana (ecotype Col-0) with mutant viruses and by expression of mutant 2b transgenes in A. thaliana. We confirmed that two nuclear localization signals were required for symptom induction and found that the N-terminal domain was essential for symptom induction. The C-terminal domain and two serine residues within a putative phosphorylation domain modulated symptom severity. Further infection studies were conducted using Fny-CMVΔ2b, a mutant that cannot express the 2b protein and that induces no symptoms in N. tabacum, N. benthamiana, or A. thaliana ecotype Col-0. Surprisingly, in plants of A. thaliana ecotype C24, Fny-CMVΔ2b induced severe symptoms similar to those induced by the wild-type virus. However, C24 plants infected with the mutant virus recovered from disease while those infected with the wild-type virus did not. Expression of 2b transgenes from either Fny-CMV or from LS-CMV (a mild strain) in Col-0 plants enhanced systemic movement of Fny-CMVΔ2b and permitted symptom induction by Fny-CMVΔ2b. Taken together, the results indicate that the 2b protein itself is an important symptom determinant in certain hosts. However, they also suggest that the protein may somehow synergize symptom induction by other CMV-encoded factors.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology Q Science > QR Microbiology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Warwick HRI (2004-2010)
Library of Congress Subject Headings (LCSH):	Cucumber mosaic virus, Viral proteins, Virus diseases of plants -- Genetic aspects, Arabidopsis thaliana -- Diseases and pests, Tobacco -- Diseases and pests
Journal or Publication Title:	Molecular Plant - Microbe Interactions
Publisher:	American Phytopathological Society
ISSN:	0894-0282
Date:	June 2009
Volume:	Vol.22
Number:	No.6
Page Range:	pp. 642-654
Identification Number:	10.1094/MPMI-22-6-0642
Status:	Peer Reviewed
Access rights to Published version:	Open Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Great Britain. Dept. for Environment, Food & Rural Affairs (DEFRA), Scotland. Rural and Environment Research and Analysis Directorate (RERAD), Plant Signaling Network Research Center (Korea University) (PSNRC), Korean Science and Engineering Fund (KOSEF), Gatsby Charitable Foundation (GCF), Fondation Baxter et Alma Ricard (FBAR), Trinity Hall (University of Cambridge)
Grant number:	HH3205SFV (DEFRA), BB/D008204/1 (BBSRC), R11-2003-008-02002-0 (PSNRC), KRF-2005-214-C00156 (KOSEF)
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URI:	http://wrap.warwick.ac.uk/id/eprint/731