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A simple disc diffusion method for detecting AmpC and extended-spectrum -lactamases in clinical isolates of Enterobacteriaceae
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Derbyshire, H., Kay, Gemma L., Evans, K., Vaughan, C., Kavuri, U. and Winstanley, T. (2009) A simple disc diffusion method for detecting AmpC and extended-spectrum -lactamases in clinical isolates of Enterobacteriaceae. Journal of Antimicrobial Chemotherapy, 63 (3). pp. 497-501. doi:10.1093/jac/dkn535 ISSN 0305-7453.
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Official URL: http://dx.doi.org/10.1093/jac/dkn535
Abstract
Background: We sought to determine whether extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases (derepressed and inducible), alone and in combination, could be detected in unidentified members of the Enterobacteriaceae using a simple, overnight disc diffusion test.
Methods: The genetic basis of antibiotic resistance in cephalosporin-resistant wild-type (n = 140) and culture collection (n = 140) isolates of Enterobacteriaceae was determined using PCR. A scheme for detecting these resistance mechanisms phenotypically was devised using five antibiotic discs: cefpodoxime ± clavulanate; cefepime ± clavulanate and cefoxitin.
Results and conclusions: AmpC β-lactamases (derepressed and inducible) and ESBLs, alone and in combination, could reliably be detected using a disc diffusion method. ESBLs alone could be detected on the basis of a difference of >5 mm between cefpodoxime/clavulanate and cefpodoxime (10 µg) discs. ESBLs, in the presence of AmpC β-lactamases, could be detected using a difference of >5 mm between cefepime/clavulanate and cefepime (30 µg) discs. AmpC β-lactamases could be detected using a difference of >14 mm between cefepime/clavulanate and cefpodoxime/clavulanate discs. Inducible AmpC β-lactamases could be discerned by observing the blunting of the cefpodoxime or cefpodoxime/clavulanate zones in proximity to cefoxitin (30 µg) discs.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Journal of Antimicrobial Chemotherapy | ||||
| Publisher: | Oxford University Press | ||||
| ISSN: | 0305-7453 | ||||
| Official Date: | 2009 | ||||
| Dates: |
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| Volume: | 63 | ||||
| Number: | 3 | ||||
| Page Range: | pp. 497-501 | ||||
| DOI: | 10.1093/jac/dkn535 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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