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Accurate determination of microbial diversity from 454 pyrosequencing data
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Quince, Christopher, Lanzén, Anders, Curtis, Thomas P., Davenport, Russell J., Hall, Neil, Head, Ian M., Read, L Fiona and Sloan, William T. (2009) Accurate determination of microbial diversity from 454 pyrosequencing data. Nature Methods, 6 (9). pp. 639-641. doi:10.1038/nmeth.1361
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Official URL: http://dx.doi.org/10.1038/nmeth.1361
Abstract
We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.
Item Type: | Journal Item | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
Journal or Publication Title: | Nature Methods | ||||
ISSN: | 1548-7091 | ||||
Official Date: | 2009 | ||||
Dates: |
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Volume: | 6 | ||||
Number: | 9 | ||||
Page Range: | pp. 639-641 | ||||
DOI: | 10.1038/nmeth.1361 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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