Frenguelli, Bruno G. and Wall, Mark J. (2016) Combined electrophysiological and biosensor approaches to study purinergic regulation of epileptiform activity in cortical tissue. Journal of Neuroscience Methods, 260 . pp. 202-214. doi:10.1016/j.jneumeth.2015.09.011 ISSN 0165-0270.
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Abstract
Background:
Cortical brain slices offer a readily accessible experimental model of a region of the brain commonly affected by epilepsy. The diversity of recording techniques, seizure-promoting protocols and mutant mouse models provides a rich diversity of avenues of investigation, which is facilitated by the regular arrangement of distinct neuronal populations and afferent fibre pathways, particularly in the hippocampus.
New method and results:
We have been interested in the regulation of seizure activity in hippocampal and neocortical slices by the purines, adenosine and ATP. Via the use of microelectrode biosensors we have been able to measure the release of these important neuroactive compounds simultaneously with on-going epileptiform activity, even of brief durations. In addition, detailed numerical analysis and computational modelling has produced new insights into the kinetics and spatial distribution of elevations in purine concentration that occur during seizure activity.
Comparison and conclusions:
Such an approach allows the spatio-temporal characteristics of neurotransmitter/neuromodulator release to be directly correlated with electrophysiological measures of synaptic and seizure activity, and can provide greater insight into the role of purines in epilepsy.
| Item Type: | Journal Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) R Medicine > RC Internal medicine |
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
| Library of Congress Subject Headings (LCSH): | Epilepsy, Convulsions, Biosensors, Adenosine |
| Journal or Publication Title: | Journal of Neuroscience Methods |
| Publisher: | Elsevier BV |
| ISSN: | 0165-0270 |
| Official Date: | 15 February 2016 |
| Dates: | Date Event 15 February 2016 Published 14 September 2015 Available 7 September 2015 Accepted 20 May 2015 Submitted |
| Volume: | 260 |
| Page Range: | pp. 202-214 |
| DOI: | 10.1016/j.jneumeth.2015.09.011 |
| Status: | Peer Reviewed |
| Publication Status: | Published |
| Access rights to Published version: | Restricted or Subscription Access |
| Date of first compliant deposit: | 31 December 2015 |
| Date of first compliant Open Access: | 14 September 2016 |
| Funder: | Epilepsy Research UK |
| URI: | https://wrap.warwick.ac.uk/73265/ |
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