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Combined electrophysiological and biosensor approaches to study purinergic regulation of epileptiform activity in cortical tissue

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Frenguelli, Bruno G. and Wall, Mark J. (2016) Combined electrophysiological and biosensor approaches to study purinergic regulation of epileptiform activity in cortical tissue. Journal of Neuroscience Methods, 260 . pp. 202-214. doi:10.1016/j.jneumeth.2015.09.011

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Official URL: http://dx.doi.org/10.1016/j.jneumeth.2015.09.011

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Abstract

Background:
Cortical brain slices offer a readily accessible experimental model of a region of the brain commonly affected by epilepsy. The diversity of recording techniques, seizure-promoting protocols and mutant mouse models provides a rich diversity of avenues of investigation, which is facilitated by the regular arrangement of distinct neuronal populations and afferent fibre pathways, particularly in the hippocampus.

New method and results:
We have been interested in the regulation of seizure activity in hippocampal and neocortical slices by the purines, adenosine and ATP. Via the use of microelectrode biosensors we have been able to measure the release of these important neuroactive compounds simultaneously with on-going epileptiform activity, even of brief durations. In addition, detailed numerical analysis and computational modelling has produced new insights into the kinetics and spatial distribution of elevations in purine concentration that occur during seizure activity.

Comparison and conclusions:
Such an approach allows the spatio-temporal characteristics of neurotransmitter/neuromodulator release to be directly correlated with electrophysiological measures of synaptic and seizure activity, and can provide greater insight into the role of purines in epilepsy.

Item Type: Journal Article
Subjects: R Medicine > R Medicine (General)
R Medicine > RC Internal medicine
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Epilepsy, Convulsions, Biosensors, Adenosine
Journal or Publication Title: Journal of Neuroscience Methods
Publisher: Elsevier BV
ISSN: 0165-0270
Official Date: 15 February 2016
Dates:
DateEvent
15 February 2016Published
14 September 2015Available
7 September 2015Accepted
20 May 2015Submitted
Date of first compliant deposit: 31 December 2015
Volume: 260
Page Range: pp. 202-214
DOI: 10.1016/j.jneumeth.2015.09.011
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Epilepsy Research UK

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