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Using molecular data for epidemiological inference : assessing the prevalence of trypanosoma brucei rhodesiense in tsetse in Serengeti, Tanzania
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Kamhawi, Shaden, Auty, Harriet K., Picozzi, Kim, Malele, Imna, Torr, Stephen J., Cleaveland, Sarah and Welburn, Sue (2012) Using molecular data for epidemiological inference : assessing the prevalence of trypanosoma brucei rhodesiense in tsetse in Serengeti, Tanzania. PLoS Neglected Tropical Diseases, 6 (1). e1501. doi:10.1371/journal.pntd.0001501
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Official URL: http://dx.doi.org/10.1371/journal.pntd.0001501
Abstract
Background
Measuring the prevalence of transmissible Trypanosoma brucei rhodesiense in tsetse populations is essential for understanding transmission dynamics, assessing human disease risk and monitoring spatio-temporal trends and the impact of control interventions. Although an important epidemiological variable, identifying flies which carry transmissible infections is difficult, with challenges including low prevalence, presence of other trypanosome species in the same fly, and concurrent detection of immature non-transmissible infections. Diagnostic tests to measure the prevalence of T. b. rhodesiense in tsetse are applied and interpreted inconsistently, and discrepancies between studies suggest this value is not consistently estimated even to within an order of magnitude.
Methodology/Principal Findings
Three approaches were used to estimate the prevalence of transmissible Trypanosoma brucei s.l. and T. b. rhodesiense in Glossina swynnertoni and G. pallidipes in Serengeti National Park, Tanzania: (i) dissection/microscopy; (ii) PCR on infected tsetse midguts; and (iii) inference from a mathematical model. Using dissection/microscopy the prevalence of transmissible T. brucei s.l. was 0% (95% CI 0–0.085) for G. swynnertoni and 0% (0–0.18) G. pallidipes; using PCR the prevalence of transmissible T. b. rhodesiense was 0.010% (0–0.054) and 0.0089% (0–0.059) respectively, and by model inference 0.0064% and 0.00085% respectively.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Health Sciences > Population, Evidence & Technologies (PET) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | PLoS Neglected Tropical Diseases | ||||
| Publisher: | Public Library of Science | ||||
| ISSN: | 1935-2727 | ||||
| Official Date: | 2012 | ||||
| Dates: |
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| Volume: | 6 | ||||
| Number: | 1 | ||||
| Article Number: | e1501 | ||||
| DOI: | 10.1371/journal.pntd.0001501 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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