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Intranasal immunisation with recombinant adenovirus vaccines protects against a lethal 2 challenge with pneumonia virus of mice
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Maunder, Helen E., Taylor, Geraldine, Leppard, Keith and Easton, A. J. (Andrew J.) (2015) Intranasal immunisation with recombinant adenovirus vaccines protects against a lethal 2 challenge with pneumonia virus of mice. Vaccine, 33 (48). pp. 6641-6649. doi:10.1016/j.vaccine.2015.10.105
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Official URL: http://dx.doi.org/10.1016/j.vaccine.2015.10.105
Abstract
Pneumonia virus of mice (PVM) infection of BALB/c mice induces bronchiolitis leading to a fatal pneumonia in a dose-dependent manner, closely paralleling the development of severe disease during human respiratory syncytial virus infection in man, and is thus a recognised model in which to study the pathogenesis of pneumoviruses. This model system was used to investigate delivery of the internal structural proteins of PVM as a potential vaccination strategy to protect against pneumovirus disease. Replication-deficient recombinant human adenovirus serotype 5 (rAd5) vectors were constructed that expressed the M or N gene of PVM pathogenic strain J3666. Intranasal delivery of these rAd5 vectors gave protection against a lethal challenge dose of PVM in three different mouse strains, and protection lasted for at least 20 weeks post-immunisation. Whilst the PVM-specific antibody response isuch animals was weak and inconsistent, rAd5N primed a strong PVM-specific CD8+ T cell response and, to a lesser extent, a CD4+ T cell response. These findings suggest that protection induced by rAd5N was mediated by T-cells rather than serum antibody.
| Item Type: | Journal Article | ||||||||||
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| Subjects: | Q Science > QR Microbiology > QR355 Virology | ||||||||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||
| Library of Congress Subject Headings (LCSH): | Viral pneumonia -- Vaccination, Vaccines, Intranasal medication, Adenoviruses | ||||||||||
| Journal or Publication Title: | Vaccine | ||||||||||
| Publisher: | Elsevier Ltd. | ||||||||||
| ISSN: | 0264-410X | ||||||||||
| Official Date: | 27 November 2015 | ||||||||||
| Dates: |
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| Date of first compliant deposit: | 31 December 2015 | ||||||||||
| Volume: | 33 | ||||||||||
| Number: | 48 | ||||||||||
| Page Range: | pp. 6641-6649 | ||||||||||
| DOI: | 10.1016/j.vaccine.2015.10.105 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||
| Funder: | Medical Research Council (Great Britain) (MRC) | ||||||||||
| Related URLs: |
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