The Library
Intranasal immunisation with recombinant adenovirus vaccines protects against a lethal 2 challenge with pneumonia virus of mice
Tools
Tools
Tools
Maunder, Helen E., Taylor, Geraldine, Leppard, Keith and Easton, A. J. (Andrew J.) (2015) Intranasal immunisation with recombinant adenovirus vaccines protects against a lethal 2 challenge with pneumonia virus of mice. Vaccine, 33 (48). pp. 6641-6649. doi:10.1016/j.vaccine.2015.10.105
|
PDF
WRAP_Maunder_-jvac-d-15-01492_accepted.pdf - Accepted Version - Requires a PDF viewer. Download (2093Kb) | Preview |
|
|
PDF
WRAP_maunder_et_al_rad5_pvm_-_preprint.pdf - Submitted Version - Requires a PDF viewer. Download (1722Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.vaccine.2015.10.105
Abstract
Pneumonia virus of mice (PVM) infection of BALB/c mice induces bronchiolitis leading to a fatal pneumonia in a dose-dependent manner, closely paralleling the development of severe disease during human respiratory syncytial virus infection in man, and is thus a recognised model in which to study the pathogenesis of pneumoviruses. This model system was used to investigate delivery of the internal structural proteins of PVM as a potential vaccination strategy to protect against pneumovirus disease. Replication-deficient recombinant human adenovirus serotype 5 (rAd5) vectors were constructed that expressed the M or N gene of PVM pathogenic strain J3666. Intranasal delivery of these rAd5 vectors gave protection against a lethal challenge dose of PVM in three different mouse strains, and protection lasted for at least 20 weeks post-immunisation. Whilst the PVM-specific antibody response isuch animals was weak and inconsistent, rAd5N primed a strong PVM-specific CD8+ T cell response and, to a lesser extent, a CD4+ T cell response. These findings suggest that protection induced by rAd5N was mediated by T-cells rather than serum antibody.
| Item Type: | Journal Article | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QR Microbiology > QR355 Virology | ||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||
| Library of Congress Subject Headings (LCSH): | Viral pneumonia -- Vaccination, Vaccines, Intranasal medication, Adenoviruses | ||||||||||
| Journal or Publication Title: | Vaccine | ||||||||||
| Publisher: | Elsevier Ltd. | ||||||||||
| ISSN: | 0264-410X | ||||||||||
| Official Date: | 27 November 2015 | ||||||||||
| Dates: |
|
||||||||||
| Volume: | 33 | ||||||||||
| Number: | 48 | ||||||||||
| Page Range: | pp. 6641-6649 | ||||||||||
| DOI: | 10.1016/j.vaccine.2015.10.105 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||
| Funder: | Medical Research Council (Great Britain) (MRC) | ||||||||||
| Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
