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Defining a pipeline for metaproteomic analyses

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Christie-Oleza, Joseph Alexander, Sousoni, Despoina, Armengaud, Jean, Wellington, E. M. H. and Jones, Alexandra M. (2015) Defining a pipeline for metaproteomic analyses. Springer Protocols Handbooks . doi:10.1007/8623_2015_130

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Official URL: http://dx.doi.org/10.1007/8623_2015_130

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Abstract

Metaproteomics is the analysis of the proteome of environmental samples. While proteomics has been established as a robust and reliable technique, the meta aspect of this omic approach is still in its infancy and subject to methodological and data analysis improvements over the next years. The need to define correct methods for such analyses is essential before hypothesis-driven projects can be addressed. Here we discuss the current state of metaproteomics and propose a protocol covering the three main steps to be implemented in any metaproteomics pipeline: (1) sample preparation, (2) high-throughput mass spectrometry analysis and (3) data search. We also detail current bottlenecks and alternatives to such pipeline.

Item Type: Journal Article
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title: Springer Protocols Handbooks
Publisher: Humana Press
ISSN: 1949-2448
Official Date: 14 August 2015
Dates:
DateEvent
14 August 2015Published
DOI: 10.1007/8623_2015_130
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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