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Defining a pipeline for metaproteomic analyses
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Christie-Oleza, Joseph Alexander, Sousoni, Despoina, Armengaud, Jean, Wellington, E. M. H. and Jones, Alexandra M. (2015) Defining a pipeline for metaproteomic analyses. Springer Protocols Handbooks . doi:10.1007/8623_2015_130
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Official URL: http://dx.doi.org/10.1007/8623_2015_130
Abstract
Metaproteomics is the analysis of the proteome of environmental samples. While proteomics has been established as a robust and reliable technique, the meta aspect of this omic approach is still in its infancy and subject to methodological and data analysis improvements over the next years. The need to define correct methods for such analyses is essential before hypothesis-driven projects can be addressed. Here we discuss the current state of metaproteomics and propose a protocol covering the three main steps to be implemented in any metaproteomics pipeline: (1) sample preparation, (2) high-throughput mass spectrometry analysis and (3) data search. We also detail current bottlenecks and alternatives to such pipeline.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Springer Protocols Handbooks | ||||
| Publisher: | Humana Press | ||||
| ISSN: | 1949-2448 | ||||
| Official Date: | 14 August 2015 | ||||
| Dates: |
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| DOI: | 10.1007/8623_2015_130 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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