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Surface immobilization of a protease through an inhibitor-derived affinity ligand : a bioactive surface with defensive properties against an inhibitor
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Tang, Zengchao, Luan, Yafei, Li, Dan, Du, Hui, Haddleton, David M. and Chen, Hong (2015) Surface immobilization of a protease through an inhibitor-derived affinity ligand : a bioactive surface with defensive properties against an inhibitor. Chemical Communications, 51 (75). pp. 14263-14266. doi:10.1039/c5cc05652g ISSN 1359-7345.
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Official URL: http://dx.doi.org/10.1039/C5CC05652G
Abstract
The concept of enzyme immobilization via an inhibitor-derived peptide was developed. This method of immobilization was shown to be advantageous over physical adsorption and covalent bonding in retaining the enzymatic activity. Moreover, the surface-immobilized enzyme exhibited resistance against its inhibitor due to the occupation of an inhibitor binding site on the enzyme.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
Journal or Publication Title: | Chemical Communications | ||||||||
Publisher: | Royal Society of Chemistry | ||||||||
ISSN: | 1359-7345 | ||||||||
Official Date: | 28 September 2015 | ||||||||
Dates: |
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Volume: | 51 | ||||||||
Number: | 75 | ||||||||
Page Range: | pp. 14263-14266 | ||||||||
DOI: | 10.1039/c5cc05652g | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access |
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