Lorson, Monique A., Dickson, Alexa M., Shaw, Debra J., Todd, Adrian G., Young, Elizabeth C., Morse, Robert, Wolstencroft, Catherine, Lorson, Christian L. and Young, Philip J. (2008) Identification and characterisation of a nuclear localisation signal in the SMN associated protein, Gemin4. Biochemical and Biophysical Research Communications, 375 (1). pp. 33-37. doi:10.1016/j.bbrc.2008.07.113 ISSN 0006-291X.

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Abstract

Gemin4 is a ubiquitously expressed multifunctional protein that is involved in U snRNP assembly, apoptosis, nuclear/cytoplasmic transportation, transcription, and RNAi pathways. Gemin4 is one of the core components of the Gemin-complex, which also contains survival motor neuron (SMN), the seven Gemin proteins (Gemin2–8), and Unrip. Mutations in the SMN1 gene cause the autosomal recessive disorder spinal muscular atrophy (SMA). Although the functions assigned to Gemin4 predominantly occur in the nucleus, the mechanisms that mediate the nuclear import of Gemin4 remain unclear. Here, using a novel panel of Gemin4 constructs we identify a canonical nuclear import sequence (NLS) in the N-terminus of Gemin4. The Gemin4 NLS is necessary and independently sufficient to mediate nuclear import of Gemin4. This is the first functional NLS identified within the SMN–Gemin complex.

Item Type:	Journal Article
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
ISSN:	0006-291X
Official Date:	2008
Dates:	Date Event 2008 Published
Volume:	375
Number:	1
Page Range:	pp. 33-37
DOI:	10.1016/j.bbrc.2008.07.113
Status:	Peer Reviewed
Publication Status:	Published

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