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Gold nanoparticle aggregation as a probe of antifreeze (glyco) protein-inspired ice recrystallization inhibition and identification of new IRI active macromolecules

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Mitchell, Daniel E., Congdon, Thomas R., Rodger, Alison and Gibson, Matthew I. (2015) Gold nanoparticle aggregation as a probe of antifreeze (glyco) protein-inspired ice recrystallization inhibition and identification of new IRI active macromolecules. Scientific Reports, 5 . 15716. doi:10.1038/srep15716

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Official URL: http://dx.doi.org/10.1038/srep15716

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Abstract

Antifreeze (glyco)proteins are found in polar fish species and act to slow the rate of growth of ice crystals; a property known as ice recrystallization inhibition. The ability to slow ice growth is of huge technological importance especially in the cryopreservation of donor cells and tissue, but native antifreeze proteins are often not suitable, nor easily available. Therefore, the search for new materials that mimic this function is important, but currently limited by the low-throughout assays associated with the antifreeze properties. Here 30 nm gold nanoparticles are demonstrated to be useful colorimetric probes for ice recrystallization inhibition, giving a visible optical response and is compatible with 96 well plates for high-throughout studies. This method is faster, requires less infrastructure, and has easier interpretation than the currently used ‘splat’ methods. Using this method, a series of serum proteins were identified to have weak, but specific ice recrystallization inhibition activity, which was removed upon denaturation. It is hoped that high-throughput tools such as this will accelerate the discovery of new antifreeze mimics.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Divisions: Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH): Antifreeze proteins
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
Official Date: 25 October 2015
Dates:
DateEvent
25 October 2015Published
2 October 2015Accepted
27 April 2015Submitted
Date of first compliant deposit: 8 December 2015
Volume: 5
Article Number: 15716
DOI: 10.1038/srep15716
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
Funder: Science City Research Alliance, Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Advantage West Midlands (AWM), European Regional Development Fund (ERDF), Leverhulme Trust (LT), Royal Society (Great Britain), Engineering and Physical Sciences Research Council (EPSRC)
Grant number: BB/F011199/1 (BBSRC), RPG-144 (LT), EP/F500378/1 (EPSRC)
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