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Gold nanoparticle aggregation as a probe of antifreeze (glyco) protein-inspired ice recrystallization inhibition and identification of new IRI active macromolecules
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Mitchell, Daniel E., Congdon, Thomas R., Rodger, Alison and Gibson, Matthew I. (2015) Gold nanoparticle aggregation as a probe of antifreeze (glyco) protein-inspired ice recrystallization inhibition and identification of new IRI active macromolecules. Scientific Reports, 5 . 15716. doi:10.1038/srep15716
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Official URL: http://dx.doi.org/10.1038/srep15716
Abstract
Antifreeze (glyco)proteins are found in polar fish species and act to slow the rate of growth of ice crystals; a property known as ice recrystallization inhibition. The ability to slow ice growth is of huge technological importance especially in the cryopreservation of donor cells and tissue, but native antifreeze proteins are often not suitable, nor easily available. Therefore, the search for new materials that mimic this function is important, but currently limited by the low-throughout assays associated with the antifreeze properties. Here 30 nm gold nanoparticles are demonstrated to be useful colorimetric probes for ice recrystallization inhibition, giving a visible optical response and is compatible with 96 well plates for high-throughout studies. This method is faster, requires less infrastructure, and has easier interpretation than the currently used ‘splat’ methods. Using this method, a series of serum proteins were identified to have weak, but specific ice recrystallization inhibition activity, which was removed upon denaturation. It is hoped that high-throughput tools such as this will accelerate the discovery of new antifreeze mimics.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QD Chemistry | ||||||||
| Divisions: | Faculty of Science > Chemistry | ||||||||
| Library of Congress Subject Headings (LCSH): | Antifreeze proteins | ||||||||
| Journal or Publication Title: | Scientific Reports | ||||||||
| Publisher: | Nature Publishing Group | ||||||||
| ISSN: | 2045-2322 | ||||||||
| Official Date: | 25 October 2015 | ||||||||
| Dates: |
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| Date of first compliant deposit: | 8 December 2015 | ||||||||
| Volume: | 5 | ||||||||
| Article Number: | 15716 | ||||||||
| DOI: | 10.1038/srep15716 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access | ||||||||
| Funder: | Science City Research Alliance, Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Advantage West Midlands (AWM), European Regional Development Fund (ERDF), Leverhulme Trust (LT), Royal Society (Great Britain), Engineering and Physical Sciences Research Council (EPSRC) | ||||||||
| Grant number: | BB/F011199/1 (BBSRC), RPG-144 (LT), EP/F500378/1 (EPSRC) | ||||||||
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