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Glycosylated gold nanoparticle libraries for label-free multiplexed lectin biosensing
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Richards, Sarah-Jane, Otten, Lucienne and Gibson, Matthew I. (2015) Glycosylated gold nanoparticle libraries for label-free multiplexed lectin biosensing. Journal of Materials Chemistry B . doi:10.1039/c5tb01994j
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Official URL: http://dx.doi.org/10.1039/C5TB01994J
Abstract
Glycan/lectin interactions drive a wide range of recognition and signal transduction processes within nature. However, their measurement is complicated or limited by the analytical tools available. Most technologies require fluorescently labelled proteins (e.g. microarrays) or expensive infrastructure (such as surface plasmon resonance). This also limits their application in biosensing, especially for low-resource settings, where detection of pathogens based on glycan binding could speed up diagnosis. Here we employ a library-oriented approach to immobilise a range of monosaccharides onto polymer-stabilised gold nanoparticles to enable rapid and high-throughput evaluation of their binding specificities with a panel of lectins. The red to blue colour shift upon gold nanoparticle aggregation is used as the output, removing the need for labelled protein, enabling compatibility with 96-well microplates. Furthermore, we demonstrate the use of a flatbed scanner (or digital camera) to extract biophysical data, ensuring that only minimal resources are required. Finally, linear discriminant analysis is employed to demonstrate how the glyconanoparticles can be applied as a multiplexed biosensor capable of identifying pathogenic lectins without the need for any infrastructure and overcoming some of the issues of lectin promiscuity.
| Item Type: | Journal Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Subjects: | R Medicine > R Medicine (General) | ||||||||
| Divisions: | Faculty of Science > Chemistry | ||||||||
| Library of Congress Subject Headings (LCSH): | Biosensors | ||||||||
| Journal or Publication Title: | Journal of Materials Chemistry B | ||||||||
| Publisher: | R S C Publications | ||||||||
| ISSN: | 2050-750X | ||||||||
| Official Date: | 27 October 2015 | ||||||||
| Dates: |
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| DOI: | 10.1039/c5tb01994j | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access | ||||||||
| Funder: | Advantage West Midlands (AWM), European Regional Development Fund (ERDF), Engineering and Physical Sciences Research Council (EPSRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||
| Grant number: | EP/F500378/1 (EPSRC), BB/F011199/ 1 (BBSRC) |
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