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Quantifying cellular mechanics and adhesion in renal tubular injury using single cell force spectroscopy
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Siamantouras, Eleftherios, Hills, Claire E., Squires, Paul E. and Liu, Kuo-Kang (2016) Quantifying cellular mechanics and adhesion in renal tubular injury using single cell force spectroscopy. Nanomedicine : Nanotechnology, Biology, and Medicine, 12 (4). pp. 1013-1021. doi:10.1016/j.nano.2015.12.362
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WRAP_0874042-es-121215-00final__cell_mechanics_renal.pdf - Accepted Version - Requires a PDF viewer. Download (892Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.nano.2015.12.362
Abstract
Tubulointerstitial fibrosis represents the major underlying pathology of diabetic nephropathy where loss of cell-to-cell adhesion is a critical step. To date, research has predominantly focussed on the loss of cell surface molecular binding events that include altered protein ligation. In the current study, atomic force microscopy single cell force spectroscopy (AFM-SCFS) was used to quantify changes in cellular stiffness and cell adhesion in TGF-β1 treated kidney cells of the human proximal tubule (HK2). AFM indentation of TGF-β1 treated HK2 cells showed a significant increase (42%) in the elastic modulus (stiffness) compared to control. Fluorescence microscopy confirmed that increased cell stiffness is accompanied by reorganization of the cytoskeleton. The corresponding changes in stiffness, due to F-actin rearrangement, affected the work of detachment by changing the separation distance between two adherent cells. Overall, our novel data quantitatively demonstrate a correlation between cellular elasticity, adhesion and early morphologic/phenotypic changes associated with tubular injury.
| Item Type: | Journal Article | ||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QP Physiology R Medicine > RB Pathology T Technology > TK Electrical engineering. Electronics Nuclear engineering |
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| Divisions: | Faculty of Science > Engineering | ||||||||||
| Library of Congress Subject Headings (LCSH): | Atomic force microscopy, Fibrosis, Nanostructured materials -- Mechanical properties, Cells -- Elastic properties, Kidney tubules, Transforming growth factors-beta | ||||||||||
| Journal or Publication Title: | Nanomedicine : Nanotechnology, Biology, and Medicine | ||||||||||
| Publisher: | Elsevier | ||||||||||
| ISSN: | 1549-9634 | ||||||||||
| Official Date: | May 2016 | ||||||||||
| Dates: |
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| Volume: | 12 | ||||||||||
| Number: | 4 | ||||||||||
| Number of Pages: | 26 | ||||||||||
| Page Range: | pp. 1013-1021 | ||||||||||
| DOI: | 10.1016/j.nano.2015.12.362 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||
| Funder: | Diabetes UK, Leverhulme Trust (LT), European Foundation for the Study of Diabetes (EFSD) | ||||||||||
| Grant number: | BDA: 12/0004546 (Diabetes UK) , PRG-2012-738 (LT) | ||||||||||
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