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Quantifying cellular mechanics and adhesion in renal tubular injury using single cell force spectroscopy

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Siamantouras, Eleftherios, Hills, Claire E., Squires, Paul E. and Liu, Kuo-Kang (2016) Quantifying cellular mechanics and adhesion in renal tubular injury using single cell force spectroscopy. Nanomedicine : Nanotechnology, Biology, and Medicine, 12 (4). pp. 1013-1021. doi:10.1016/j.nano.2015.12.362

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Official URL: http://dx.doi.org/10.1016/j.nano.2015.12.362

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Abstract

Tubulointerstitial fibrosis represents the major underlying pathology of diabetic nephropathy where loss of cell-to-cell adhesion is a critical step. To date, research has predominantly focussed on the loss of cell surface molecular binding events that include altered protein ligation. In the current study, atomic force microscopy single cell force spectroscopy (AFM-SCFS) was used to quantify changes in cellular stiffness and cell adhesion in TGF-β1 treated kidney cells of the human proximal tubule (HK2). AFM indentation of TGF-β1 treated HK2 cells showed a significant increase (42%) in the elastic modulus (stiffness) compared to control. Fluorescence microscopy confirmed that increased cell stiffness is accompanied by reorganization of the cytoskeleton. The corresponding changes in stiffness, due to F-actin rearrangement, affected the work of detachment by changing the separation distance between two adherent cells. Overall, our novel data quantitatively demonstrate a correlation between cellular elasticity, adhesion and early morphologic/phenotypic changes associated with tubular injury.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QP Physiology
R Medicine > RB Pathology
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Divisions: Faculty of Science > Engineering
Library of Congress Subject Headings (LCSH): Atomic force microscopy, Fibrosis, Nanostructured materials -- Mechanical properties, Cells -- Elastic properties, Kidney tubules, Transforming growth factors-beta
Journal or Publication Title: Nanomedicine : Nanotechnology, Biology, and Medicine
Publisher: Elsevier
ISSN: 1549-9634
Official Date: May 2016
Dates:
DateEvent
May 2016Published
28 December 2015Available
2 December 2015Accepted
12 July 2015Submitted
Volume: 12
Number: 4
Number of Pages: 26
Page Range: pp. 1013-1021
DOI: 10.1016/j.nano.2015.12.362
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Diabetes UK, Leverhulme Trust (LT), European Foundation for the Study of Diabetes (EFSD)
Grant number: BDA: 12/0004546 (Diabetes UK) , PRG-2012-738 (LT)
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