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Use of REMI and Agrobacterium-mediated transformation to identify pathogenicity mutants of the biocontrol fungus, Coniothyrium minitans
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UNSPECIFIED. (2004) Use of REMI and Agrobacterium-mediated transformation to identify pathogenicity mutants of the biocontrol fungus, Coniothyrium minitans. FEMS MICROBIOLOGY LETTERS, 241 (2). pp. 207-214. ISSN 0378-1097
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Official URL: http://dx.doi.org/10.1016/j.femsle.2004.10.022
Abstract
Restriction enzyme mediated integration (REMI) and Agrobacterium-mediated transformation (ATMT) were used to transform protoplasts or germinated conidia of the mycoparasite Coniothyrium minitans to hygromycin resistance. Using REMI, up to 32 transformants mug DNA(-1) were obtained, while 37.8 transformants 5 x 10(5) germlings(-1) were obtained using ATMT. Single-copy integrations occurred in 8% and 40% of REMI and ATMT transformants, respectively. A novel microtitre plate-based test was developed to expedite screening of 4000 REMI and ATMT C minitans transformants. Nine pathogenicity mutants that displayed reduced or no pathogenicity on sclerotia of Sclerotinia sclerotiorum were identified. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Journal or Publication Title: | FEMS MICROBIOLOGY LETTERS |
| Publisher: | ELSEVIER SCIENCE BV |
| ISSN: | 0378-1097 |
| Date: | 15 December 2004 |
| Volume: | 241 |
| Number: | 2 |
| Number of Pages: | 8 |
| Page Range: | pp. 207-214 |
| Identification Number: | 10.1016/j.femsle.2004.10.022 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/7526 |
Data sourced from Thomson Reuters' Web of Knowledge
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