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Survival of the ovine footrot pathogen Dichelobacter nodosus in different soils
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Muzafar, Mohd, Green, Laura E., Calvo-Bado, Leo A., Tichauer, Esther, King, Hayley C., James, Philip and Wellington, E. M. H. (2015) Survival of the ovine footrot pathogen Dichelobacter nodosus in different soils. Anaerobe, 38 . pp. 81-87. doi:10.1016/j.anaerobe.2015.12.010 ISSN 10759964.
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Official URL: http://dx.doi.org/10.1016/j.anaerobe.2015.12.010
Abstract
Dichelobacter nodosus (D. nodosus) is the causative agent of footrot in sheep; one of the most important health and welfare issues of sheep worldwide. For control programmes to be effective, it is essential that the transmission cycle of D. nodosus is understood and bacterial reservoirs in the environment are better defined. This study evaluated the survival of D. nodosus in different soils using soil microcosms. Cultivation independent and dependent methods were used to detect D. nodosus over 40 days from seeding in soil. A D. nodosus specific probe was used for quantification by qPCR and viability was assessed by cell permeability to an intercalating dye, PMA, and by culture. Survival varied dramatically depending on soil type, matric potential (MP) and temperature. Our findings indicate that D. nodosus survival was higher at 5 °C compared with 25 °C in all soils and significantly longer at both temperatures in clay soil (>44% clay) compared with other soil types. Survival under all conditions was longer than 30 days for both culture independent and dependent methods, this is substantially longer than previous studies and, if this is an infectious dose, longer than the current recommendation of resting a field for 14 days to prevent onward infection.
Item Type: | Journal Article | ||||||||
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Subjects: | S Agriculture > SF Animal culture | ||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Library of Congress Subject Headings (LCSH): | Sheep -- Diseases, Footrot in sheep, Dichelobacter nodosus, Veterinary epidemiology, Ruminants -- Diseases | ||||||||
Journal or Publication Title: | Anaerobe | ||||||||
Publisher: | Elsevier BV | ||||||||
ISSN: | 10759964 | ||||||||
Official Date: | 30 December 2015 | ||||||||
Dates: |
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Volume: | 38 | ||||||||
Page Range: | pp. 81-87 | ||||||||
DOI: | 10.1016/j.anaerobe.2015.12.010 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||
Date of first compliant deposit: | 4 February 2016 | ||||||||
Date of first compliant Open Access: | 5 February 2016 | ||||||||
Funder: | Natural Environment Research Council (Great Britain) (NERC) | ||||||||
Grant number: | My3820 |
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