Mitchell, Daniel E., Lovett, Joseph R., Armes, Steven P. and Gibson, Matthew I. (2016) Combining biomimetic block copolymer worms with an ice-inhibiting polymer for the solvent-free cryopreservation of red blood cells. Angewandte Chemie International Edition, 128 (8). pp. 2851-2854. doi:10.1002/anie.201511454 ISSN 1433-7851.

Preview

PDF WRAP_Mitchell_et_al-2016-Angewandte_Chemie_International_Edition (1).pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2386Kb) | Preview

Request Changes to record.

Abstract

The first fully synthetic polymer-based approach for red-blood-cell cryopreservation without the need for any (toxic) organic solvents is reported. Highly hydroxylated block copolymer worms are shown to be a suitable replacement for hydroxyethyl starch as a extracellular matrix for red blood cells. When used alone, the worms are not a particularly effective preservative. However, when combined with poly(vinyl alcohol), a known ice-recrystallization inhibitor, a remarkable additive cryopreservative effect is observed that matches the performance of hydroxyethyl starch. Moreover, these block copolymer worms enable post-thaw gelation by simply warming to 20 °C. This approach offers a new solution for both the storage and transport of red blood cells and also a convenient matrix for subsequent 3D cell cultures.

Item Type:	Journal Article
Subjects:	R Medicine > R Medicine (General)
Divisions:	Faculty of Science, Engineering and Medicine > Science > Chemistry
Library of Congress Subject Headings (LCSH):	Biomedical materials, Block copolymers, Cryopreservation of organs, tissues, etc, Freeze-drying -- Methods, Frozen blood, Cryobiology, Micelles
Journal or Publication Title:	Angewandte Chemie International Edition
Publisher:	Wiley - V C H Verlag GmbH & Co. KGaA
ISSN:	1433-7851
Official Date:	8 February 2016
Dates:	Date Event 8 February 2016 Published 28 January 2016 Available 2015 Accepted 10 December 2015 Submitted
Volume:	128
Number:	8
Page Range:	pp. 2851-2854
DOI:	10.1002/anie.201511454
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Date of first compliant deposit:	11 February 2016
Date of first compliant Open Access:	12 February 2016
Funder:	Engineering and Physical Sciences Research Council (EPSRC), Molecular Organisation and Assembly in Cells (MOAC) Doctoral Training Centre, European Research Council (ERC)
Grant number:	EP/F500378/1, PISA 320372, EP/L024160/1, CRYOMAT 638661

Request changes or add full text files to a record

Repository staff actions (login required)

View Item

Downloads