UNSPECIFIED. (2004) Genetic dissection of the light-inducible carQRS promoter region of Myxococcus xanthus. JOURNAL OF BACTERIOLOGY, 186 (23). pp. 7836-7846. ISSN 0021-9193

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Abstract

In Myxococcus xanthus photoprotective carotenoids are produced in response to illumination due to regulated expression of carotenoid biosynthesis genes at two loci. Induction of the carotenogenesis regulon is dependent on expression of the carQRS operon. The first gene product of the operon, CarQ, is a sigma factor belonging to the ECF family and is responsible for light-dependent initiation of transcription at the carQRS promoter. We defined the minimal carQRS promoter as a 145-bp fragment of DNA upstream of the carQRS transcriptional start site, which includes the promoter for a divergent gene, gufA. In order to elucidate regions with the promoter required for activity, point mutations were introduced into the carQRS promoter between positions -151 and 6. While most sequence changes abolished carQRS promoter activity, two changes enhanced promoter activity and two changes caused the mutant promoter to become constitutive and independent of CarQ. The promoter-null point mutations and 6-bp deletion mutations implied that the carQRS promoter requires a functional gufA, promoter for transcriptional activity and vice versa. By mapping the extent of the promoter region, identifying sequences important for promoter activity, and highlighting potential topological effects, we provide a foundation for further analysis of the carQRS promoter.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology
Journal or Publication Title:	JOURNAL OF BACTERIOLOGY
Publisher:	AMER SOC MICROBIOLOGY
ISSN:	0021-9193
Date:	December 2004
Volume:	186
Number:	23
Number of Pages:	11
Page Range:	pp. 7836-7846
Identification Number:	10.1128/JB.186.23.7836-7846.2004
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/7746

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