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Structure of thermobifida fusca DyP-type peroxidase and activity towards kraft lignin and lignin model compounds
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Rahmanpour, Rahman , Rea, Dean, Jamshidi, Shirin, Fülöp, Vilmos and Bugg, Tim (2016) Structure of thermobifida fusca DyP-type peroxidase and activity towards kraft lignin and lignin model compounds. Archives of Biochemistry and Biophysics, 594 . pp. 54-60. doi:10.1016/j.abb.2016.02.019 ISSN 0003-9861.
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Official URL: http://dx.doi.org/10.1016/j.abb.2016.02.019
Abstract
A Dyp-type peroxidase enzyme from thermophilic cellulose degrader Thermobifida fusca (TfuDyP) was investigated for catalytic ability towards lignin oxidation. TfuDyP was characterised kinetically against a range of phenolic substrates, and a compound I reaction intermediate was observed via pre-steady state kinetic analysis at max 404 nm. TfuDyP showed reactivity towards Kraft lignin, and was found to oxidise a -aryl ether lignin model compound, forming an oxidised dimer. A crystal structure of TfuDyP was determined, to 1.8Å resolution, which was found to contain a diatomic oxygen ligand bound to the heme centre, positioned close to active site residues Asp-203 and Arg-315. The structure contains two channels providing access to the heme cofactor for organic substrates and hydrogen peroxide. Site-directed mutant D203A showed no activity towards phenolic substrates, but reduced activity towards ABTS, while mutant R315Q showed no activity towards phenolic substrates, nor ABTS.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QC Physics Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) |
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Library of Congress Subject Headings (LCSH): | Physical biochemistry, Biology -- Classification, Bacteria, Fungi, Ligninase, Phenols, Aspartic acid | ||||||||||
Journal or Publication Title: | Archives of Biochemistry and Biophysics | ||||||||||
Publisher: | Academic Press | ||||||||||
ISSN: | 0003-9861 | ||||||||||
Official Date: | 15 March 2016 | ||||||||||
Dates: |
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Volume: | 594 | ||||||||||
Page Range: | pp. 54-60 | ||||||||||
DOI: | 10.1016/j.abb.2016.02.019 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Date of first compliant deposit: | 8 March 2016 | ||||||||||
Date of first compliant Open Access: | 18 February 2017 | ||||||||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||||||||
Grant number: | BB/M025772/1 and BB/M003523/1 |
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