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Label-free imaging of microtubules with sub-nm precision using interferometric scattering microscopy
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Andrecka, Joanna, Ortega Arroyo, Jaime, Lewis, Katie, Cross, R. A. and Kukura, Philipp (2016) Label-free imaging of microtubules with sub-nm precision using interferometric scattering microscopy. Biophysical Journal, 110 (1). pp. 214-217. doi:10.1016/j.bpj.2015.10.055 ISSN 0006-3495.
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Official URL: http://dx.doi.org/10.1016/j.bpj.2015.10.055
Abstract
Current in vitro optical studies of microtubule dynamics tend to rely on fluorescent labeling of tubulin, with tracking accuracy thereby limited by the quantum yield of fluorophores and by photobleaching. Here, we demonstrate label-free tracking of microtubules with nanometer precision at kilohertz frame rates using interferometric scattering microscopy (iSCAT). With microtubules tethered to a glass substrate using low-density kinesin, we readily detect sequential 8 nm steps in the microtubule center of mass, characteristic of a single kinesin molecule moving a microtubule. iSCAT also permits dynamic changes in filament length to be measured with <5 nm precision. Using the arbitrarily long observation time enabled by label-free iSCAT imaging, we demonstrate continuous monitoring of microtubule disassembly over a 30 min period. The ability of iSCAT to track microtubules with nm precision together with its potential for label-free single protein detection and simultaneous single molecule fluorescence imaging represent a unique platform for novel approaches to studying microtubule dynamics.
| Item Type: | Journal Article | ||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Biophysical Journal | ||||||
| Publisher: | Biophysical Society | ||||||
| ISSN: | 0006-3495 | ||||||
| Official Date: | 5 January 2016 | ||||||
| Dates: |
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| Volume: | 110 | ||||||
| Number: | 1 | ||||||
| Page Range: | pp. 214-217 | ||||||
| DOI: | 10.1016/j.bpj.2015.10.055 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Restricted or Subscription Access |
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