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Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism
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Hussmann, Frauke, Drummond, Douglas R., Peet, Daniel R., Martin, Douglas S. and Cross, R. A. (2015) Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism. Scientific Reports, 6 . 20653. doi:10.1038/srep20653 ISSN 2045-2322.
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Official URL: http://dx.doi.org/10.1038/srep20653
Abstract
Alp14 is a TOG-family microtubule polymerase from S. pombe that tracks plus ends and accelerates their growth. To interrogate its mechanism, we reconstituted dynamically unstable single isoform S. pombe microtubules with full length Alp14/TOG and Alp7, the TACC-family binding partner of Alp14. We find that Alp14 can drive microtubule plus end growth at GTP-tubulin concentrations at least 10-fold below the usual critical concentration, at the expense of increased catastrophe. This reveals Alp14 to be a highly unusual enzyme that biases the equilibrium for the reaction that it catalyses. Alp7/TACC enhances the effectiveness of Alp14, by increasing its occupancy. Consistent with this, we show in live cells that Alp7 deletion produces very similar MT dynamics defects to Alp14 deletion. The ability of Alp7/14 to accelerate and bias GTP-tubulin exchange at microtubule plus ends allows it to generate long-lived, fast-growing microtubules at very low cellular free tubulin concentrations.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Cytology -- Research, Microtubules, Tubulins, Enzymes | ||||||||
| Journal or Publication Title: | Scientific Reports | ||||||||
| Publisher: | Nature Publishing Group | ||||||||
| ISSN: | 2045-2322 | ||||||||
| Official Date: | 11 February 2015 | ||||||||
| Dates: |
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| Volume: | 6 | ||||||||
| Article Number: | 20653 | ||||||||
| DOI: | 10.1038/srep20653 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 8 March 2016 | ||||||||
| Date of first compliant Open Access: | 9 March 2016 | ||||||||
| Funder: | Marie Curie Cancer Care, Wellcome Trust (London, England) | ||||||||
| Grant number: | 103895/Z/14/Z Wellcome Trust (London, England) |
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