Analysis of human GPCRs in fission yeast
UNSPECIFIED (2004) Analysis of human GPCRs in fission yeast. CURRENT OPINION IN DRUG DISCOVERY & DEVELOPMENT, 7 (5). pp. 683-691. ISSN 1367-6733Full text not available from this repository.
G protein-coupled receptors (GPCRs) regulate diverse biological processes in all eukaryotes, including yeast, insects, plants and humans. This evolutionary conservation allows an almost unrestricted interchange of signaling components between different cell types. A large number of model systems have been developed for the study of GPCRs, and yeasts provide one of the more attractive hosts since they are amenable to both genetic and biochemical manipulation, while their robustness, low cost and lack of endogenous GPCRs are ideal starting points for the development of assays suitable for high-throughput screening. The purpose of this review is to introduce readers to the possibilities of using the fission yeast Schizosaccharomyces pombe for analysis of GPCRs. We describe the endogenous signaling pathways, the development of assays for heterologous GPCRs, and some of the technology available to elucidate GPCR structure and activity.
|Item Type:||Journal Item|
|Subjects:||R Medicine > RS Pharmacy and materia medica|
|Journal or Publication Title:||CURRENT OPINION IN DRUG DISCOVERY & DEVELOPMENT|
|Number of Pages:||9|
|Page Range:||pp. 683-691|
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