Rice, Stephen J., Grant, Murray, Reynolds, Paul H. S. and Farnden, Kevin J. F. (1993) DNA sequence of Nodulin-45 from Lupinus angustifolius. Plant Science, 90 (2). pp. 155-166. doi:10.1016/0168-9452(93)90235-R ISSN 0168-9452.

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Abstract

A partial cDNA clone encoding Lupinus angustifolius Nodulin-45 was isolated by differential hybridisation. A genomic clone was also isolated, from which the DNA sequence was obtained for the 5′ end of the gene (including 1.2 kb of 5′ upstream region). The upstream region includes putative cis-elements, found upstream of other nodulin genes. Southern analysis indicates the presence of several Nodulin-45-like sequences in the lupin genome. The Nodulin-45 protein has a putative N-terminal endoplasmic reticulum-type signal sequence and also contains a large glycine-rich repeat sequence. The cDNA sequence is highly homologous to a Nodulin-45 cDNA sequence from Lupinus luteus (Szczyglowski et al., Plant Sci., 65 (1989) 87–95), although major sequence rearrangements are apparent between the L. luteus and L. angustifolius cDNAs.

Item Type:	Journal Article
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title:	Plant Science
Publisher:	Elsevier Ireland Ltd
ISSN:	0168-9452
Official Date:	1993
Dates:	Date Event 1993 Published
Volume:	90
Number:	2
Page Range:	pp. 155-166
DOI:	10.1016/0168-9452(93)90235-R
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access

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