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Molecular cloning of the gene encoding developing seed l-asparaginase from Lupinus angustifolius
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Dickson, James M. J. J., Vincze, Eva, Grant, Murray, Smith, Laura A., Rodber, Karen A., Farnden, Kevin J. F. and Reynolds, Paul H. S. (1992) Molecular cloning of the gene encoding developing seed l-asparaginase from Lupinus angustifolius. Plant Molecular Biology, 20 (2). pp. 333-336. doi:10.1007/BF00014503 ISSN 0167-4412.
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Official URL: http://dx.doi.org/10.1007/BF00014503
Abstract
A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5′ flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genomic sequence with that of a L. arboreus cDNA. Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5′ of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5′ flanking region contained sequences associated with seed-specific expression.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Plant Molecular Biology | ||||
| Publisher: | Springer | ||||
| ISSN: | 0167-4412 | ||||
| Official Date: | 1992 | ||||
| Dates: |
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| Volume: | 20 | ||||
| Number: | 2 | ||||
| Page Range: | pp. 333-336 | ||||
| DOI: | 10.1007/BF00014503 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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