The Library
Isolation of biosurfactant producers, optimization and properties of biosurfactant produced by Acinetobacter sp. from petroleum-contaminated soil
Tools
Chen, Jing, Huang, P. T., Zhang, K. Y. and Ding, F. R. (2012) Isolation of biosurfactant producers, optimization and properties of biosurfactant produced by Acinetobacter sp. from petroleum-contaminated soil. Journal of Applied Microbiology, 112 (4). pp. 660-671. doi:10.1111/j.1365-2672.2012.05242.x ISSN 1364-5072.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1111/j.1365-2672.2012.05242.x
Abstract
Aims: To screen and identify biosurfactant producers from petroleum-contaminated soil; to use response surface methodology (RSM) for medium optimization to enhance biosurfactant production; and to study the properties of the newly obtained biosurfactant towards pH, temperature and salinity.
Methods and Results: We successfully isolated three biosurfactant producers from petroleum-contaminated soil and identified them through 16S rRNA sequence analysis, which exhibit the highest similarities to Acinetobacter beijerinckii (100%), Kocuria marina (99%) and Kineococcus marinus (99%), respectively. A quadratic response model was constructed through RSM designs, leading to a 57Æ5% increase of the growth-associated biosurfactant production
by Acinetobacter sp. YC-X 2 with an optimized medium: beef extract
3Æ12 g l)1 ; peptone 20Æ87 g l)1 ; NaCl 1Æ04 g l)1 ; and n-hexadecane 1Æ86 g l)1. Biosurfactant produced by Acinetobacter sp. YC-X 2 retained its properties during exposure to a wide range of pH values (5–11), high temperatures (up to 121C) and high salinities [up to 18% (w⁄ v) Na+ and Ca2+], which was more sensitive to Ca2+ than Na+ .
Conclusions: Two novel biosurfactant producers were isolated from petroleumcontaminated soil. Biosurfactant from Acinetobacter sp. YC-X 2 has good properties to a wide range of pH, high temperature and high salinity, and its production was optimized successfully through RSM.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Journal of Applied Microbiology | ||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
ISSN: | 1364-5072 | ||||
Official Date: | 2012 | ||||
Dates: |
|
||||
Volume: | 112 | ||||
Number: | 4 | ||||
Page Range: | pp. 660-671 | ||||
DOI: | 10.1111/j.1365-2672.2012.05242.x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |