Jensen, Michael Krogh, Lindemose, Søren, de Masi, Federico, Reimer, Julia J., Nielsen, Michael, Perera, Venura, Workman, Chris T., Turck, Franziska, Grant, Murray, Mundy, John, Petersen, Morten and Skriver, Karen (2013) ATAF1 transcription factor directly regulates abscisic acid biosynthetic geneNCED3 in Arabidopsis thaliana. FEBS Open Bio, 3 (1). pp. 321-327. doi:10.1016/j.fob.2013.07.006 ISSN 2211-5463.

Research output not available from this repository.

Request-a-Copy directly from author or use local Library Get it For Me service.

Request Changes to record.

Abstract

ATAF1, an Arabidopsis thaliana NAC transcription factor, plays important roles in plant adaptation to environmental stress and development. To search for ATAF1 target genes, we used protein binding microarrays and chromatin-immunoprecipitation (ChIP). This identified T[A,C,G]CGT[A,G] and TT[A,C,G]CGT as ATAF1 consensus binding sequences. Co-expression analysis across publicly available microarray experiments identified 25 genes co-expressed with ATAF1. The promoter regions of ATAF1 co-expressors were significantly enriched for ATAF1 binding sites, and TTGCGTA was identified in the promoter of the key abscisic acid (ABA) phytohormone biosynthetic gene NCED3. ChIP-qPCR and expression analysis showed that ATAF1 binding to the NCED3 promoter correlated with increased NCED3 expression and ABA hormone levels. These results indicate that ATAF1 regulates ABA biosynthesis.

Item Type:	Journal Article
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title:	FEBS Open Bio
Publisher:	John Wiley & Sons Ltd.
ISSN:	2211-5463
Official Date:	2013
Dates:	Date Event 2013 Published
Volume:	3
Number:	1
Page Range:	pp. 321-327
DOI:	10.1016/j.fob.2013.07.006
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access

Request changes or add full text files to a record

Repository staff actions (login required)

View Item