Wasukira, Arthur, Tayebwa, Johnbosco, Thwaites, Richard, Paszkiewicz, Konrad, Aritua, Valente, Kubiriba, Jerome, Smith, Julian, Grant, Murray and Studholme, David J. (2012) Genome-wide sequencing reveals two major sub-lineages in the genetically monomorphic pathogen xanthomonas campestris pathovar musacearum. Genes, 3 (4). pp. 361-377. doi:10.3390/genes3030361 ISSN 2073-4425.

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Abstract

The bacterium Xanthomonas campestris pathovar musacearum (Xcm) is the causal agent of banana Xanthomonas wilt (BXW). This disease has devastated economies based on banana and plantain crops (Musa species) in East Africa. Here we use genome-wide sequencing to discover a set of single-nucleotide polymorphisms (SNPs) among East African isolates of Xcm. These SNPs have potential as molecular markers for phylogeographic studies of the epidemiology and spread of the pathogen. Our analysis reveals two major sub-lineages of the pathogen, suggesting that the current outbreaks of BXW on Musa species in the region may have more than one introductory event, perhaps from Ethiopia. Also, based on comparisons of genome-wide sequence data from multiple isolates of Xcm and multiple strains of X. vasicola pathovar vasculorum, we identify genes specific to Xcm that could be used to specifically detect Xcm by PCR-based methods.

Item Type:	Journal Article
Divisions:	Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title:	Genes
Publisher:	M D P I AG
ISSN:	2073-4425
Official Date:	2012
Dates:	Date Event 2012 Published
Volume:	3
Number:	4
Page Range:	pp. 361-377
DOI:	10.3390/genes3030361
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Open Access Version:	Publisher

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