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A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress
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Forcat, Silvia, Bennett, Mark H., Mansfield, John W. and Grant, Murray (2008) A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress. Plant Methods, 4 (1). p. 16. doi:10.1186/1746-4811-4-16
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Official URL: http://dx.doi.org/10.1186/1746-4811-4-16
Abstract
We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid in Arabidopsis leaf tissue.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Plant Methods | ||||
| Publisher: | BioMed Central Ltd. | ||||
| ISSN: | 1746-4811 | ||||
| Official Date: | 2008 | ||||
| Dates: |
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| Volume: | 4 | ||||
| Number: | 1 | ||||
| Page Range: | p. 16 | ||||
| DOI: | 10.1186/1746-4811-4-16 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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