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A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress

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Forcat, Silvia, Bennett, Mark H., Mansfield, John W. and Grant, Murray (2008) A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress. Plant Methods, 4 (1). p. 16. doi:10.1186/1746-4811-4-16 ISSN 1746-4811.

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Official URL: http://dx.doi.org/10.1186/1746-4811-4-16

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Abstract

We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid in Arabidopsis leaf tissue.

Item Type: Journal Article
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title: Plant Methods
Publisher: BioMed Central Ltd.
ISSN: 1746-4811
Official Date: 2008
Dates:
DateEvent
2008Published
Volume: 4
Number: 1
Page Range: p. 16
DOI: 10.1186/1746-4811-4-16
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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