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Characterisation of recombinant epithiospecifier protein and its over-expression in Arabidopsis thaliana
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Zabala, Marta de Torres, Grant, Murray, Bones, Atle M., Bennett, Richard, Lim, Yin Sze, Kissen, Ralph and Rossiter, John T. (2005) Characterisation of recombinant epithiospecifier protein and its over-expression in Arabidopsis thaliana. Phytochemistry, 66 (8). pp. 859-867. doi:10.1016/j.phytochem.2005.02.026
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Official URL: http://dx.doi.org/10.1016/j.phytochem.2005.02.026
Abstract
Epithiospecifier protein (ESP) is a protein that catalyses formation of epithionitriles during glucosinolate hydrolysis. In vitro assays with a recombinant ESP showed that the formation of epithionitriles from alkenylglucosinolates is ESP and ferrous ion dependent. Nitrile formation in vitro however does not require ESP but only the presence of Fe(II) and myrosinase. Ectopic expression of ESP in Arabidopsis thaliana Col-5 under control of the strong viral CaMV 35S promoter altered the glucosinolate product profile from isothiocyanates towards the corresponding nitriles.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Phytochemistry | ||||
| Publisher: | Pergamon Press | ||||
| ISSN: | 0031-9422 | ||||
| Official Date: | 2005 | ||||
| Dates: |
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| Volume: | 66 | ||||
| Number: | 8 | ||||
| Page Range: | pp. 859-867 | ||||
| DOI: | 10.1016/j.phytochem.2005.02.026 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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