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Biophoton imaging : a nondestructive method for assaying RGene responses

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Bennett, Mark, Mehta, Monaz and Grant, Murray (2005) Biophoton imaging : a nondestructive method for assaying RGene responses. Molecular Plant-Microbe Interactions, 18 (2). pp. 95-102. doi:10.1094/MPMI-18-0095 ISSN 0894-0282.

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Official URL: http://dx.doi.org/10.1094/MPMI-18-0095

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Abstract

Plant disease resistance (R) proteins of the nucleotide binding-leucine rich repeat class are responsible for pathogen recognition and activation of defense signaling networks leading to the hypersensitive response (HR). Genetically, R-protein signaling appears to be integrated through a limited set of common downstream components. However, the timing of development of visible HR is unique to individual R proteins. By utilizing the phenomena of ultraweak photon emission from leaves undergoing an incompatible interacttion, a powerful nondestructive and facile assay is described to compare timing of defense responses elicited by different R proteins. We demonstrate that ultraweak photon emission, or “biophoton generation,” is demonstrated to be associated with hypersensitive cell death. Biophoton emission requires an intact R signaling network and increases in cytosolic calcium and nitric oxide, but elevated reactive oxygen species are not necessary. Importantly, the assay is robust and applicable to a range of incompatible interactions in various plant species. The ability to assay R responses nondestructively in real time and a chosen genetic background makes this technique amenable to subtle genetic dissection of plant defense responses.

Item Type: Journal Article
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title: Molecular Plant-Microbe Interactions
Publisher: American Phytopathological Society
ISSN: 0894-0282
Official Date: 2005
Dates:
DateEvent
2005Published
Volume: 18
Number: 2
Page Range: pp. 95-102
DOI: 10.1094/MPMI-18-0095
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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