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Biophoton imaging : a nondestructive method for assaying RGene responses
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Bennett, Mark, Mehta, Monaz and Grant, Murray (2005) Biophoton imaging : a nondestructive method for assaying RGene responses. Molecular Plant-Microbe Interactions, 18 (2). pp. 95-102. doi:10.1094/MPMI-18-0095
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Official URL: http://dx.doi.org/10.1094/MPMI-18-0095
Abstract
Plant disease resistance (R) proteins of the nucleotide binding-leucine rich repeat class are responsible for pathogen recognition and activation of defense signaling networks leading to the hypersensitive response (HR). Genetically, R-protein signaling appears to be integrated through a limited set of common downstream components. However, the timing of development of visible HR is unique to individual R proteins. By utilizing the phenomena of ultraweak photon emission from leaves undergoing an incompatible interacttion, a powerful nondestructive and facile assay is described to compare timing of defense responses elicited by different R proteins. We demonstrate that ultraweak photon emission, or “biophoton generation,” is demonstrated to be associated with hypersensitive cell death. Biophoton emission requires an intact R signaling network and increases in cytosolic calcium and nitric oxide, but elevated reactive oxygen species are not necessary. Importantly, the assay is robust and applicable to a range of incompatible interactions in various plant species. The ability to assay R responses nondestructively in real time and a chosen genetic background makes this technique amenable to subtle genetic dissection of plant defense responses.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Molecular Plant-Microbe Interactions | ||||
| Publisher: | American Phytopathological Society | ||||
| ISSN: | 0894-0282 | ||||
| Official Date: | 2005 | ||||
| Dates: |
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| Volume: | 18 | ||||
| Number: | 2 | ||||
| Page Range: | pp. 95-102 | ||||
| DOI: | 10.1094/MPMI-18-0095 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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