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Surface charge visualization at viable living cells

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Perry, David, Paulose Nadappuram, Binoy, Momotenko, Dmitry, Voyias, Philip D., Page, Ashley M., Tripathi, Gyanendra, Frenguelli, Bruno G. and Unwin, Patrick R. (2016) Surface charge visualization at viable living cells. Journal of the American Chemical Society, 138 (9). pp. 3152-3160. doi:10.1021/jacs.5b13153 ISSN 0002-7863.

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Official URL: http://dx.doi.org/10.1021/jacs.5b13153

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Abstract

Scanning ion conductance microscopy (SICM) is demonstrated to be a powerful technique for quantitative nanoscale surface charge mapping of living cells. Utilizing a bias modulated (BM) scheme, in which the potential between a quasi-reference counter electrode (QRCE) in an electrolyte-filled nanopipette and a QRCE in bulk solution is modulated, it is shown that both the cell topography and the surface charge present at cellular interfaces can be measured simultaneously at high spatial resolution with dynamic potential measurements. Surface charge is elucidated by probing the properties of the diffuse double layer (DDL) at the cellular interface, and the technique is sensitive at both low-ionic strength and under typical physiological (high-ionic strength) conditions. The combination of experiments that incorporate pixel-level self-referencing (calibration) with a robust theoretical model allows for the analysis of local surface charge variations across cellular interfaces, as demonstrated on two important living systems. First, charge mapping at Zea mays root hairs shows that there is a high negative surface charge at the tip of the cell. Second, it is shown that there are distinct surface charge distributions across the surface of human adipocyte cells, whose role is the storage and regulation of lipids in mammalian systems. These are new features, not previously recognized, and their implications for the functioning of these cells are highlighted.

Item Type: Journal Article
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016)
Faculty of Science, Engineering and Medicine > Research Centres > Molecular Organisation and Assembly in Cells (MOAC)
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Journal or Publication Title: Journal of the American Chemical Society
Publisher: American Chemical Society
ISSN: 0002-7863
Official Date: 12 February 2016
Dates:
DateEvent
12 February 2016Published
16 December 2015Submitted
Volume: 138
Number: 9
Page Range: pp. 3152-3160
DOI: 10.1021/jacs.5b13153
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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