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Labour is associated with increased expression of type-IIA secretory phospholipase A(2) but not type-IV cytosolic phospholipase A(2) in human myometrium
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UNSPECIFIED (2004) Labour is associated with increased expression of type-IIA secretory phospholipase A(2) but not type-IV cytosolic phospholipase A(2) in human myometrium. MOLECULAR HUMAN REPRODUCTION, 10 (11). pp. 799-805. doi:10.1093/molehr/gah103 ISSN 1360-9947.
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Official URL: http://dx.doi.org/10.1093/molehr/gah103
Abstract
Human labour is associated with increased prostaglandin synthesis within the uterus. The aim of this study was to examine the expression of the type-IV cytosolic phospholipase A(2) (cPLA(2)-IV) and the type IIA secretory phospholipase A(2) (sPLA(2)-IIA) in myometrium in association with labour onset at term and preterm deliveries. These enzymes are important for the release of the prostaglandin precursor, arachidonic acid, from phospholipid membrane stores. RT-PCR was used to determine differences in gene expression between non-labour and labour groups. Expression of sPLA(2)-IIA in human myometrium was significantly increased with pregnancy, and with labour, both at term and preterm. Expression of cPLA(2)-IV in myometrium was not significantly altered with respect to pregnancy or labour. Immunohistochemical analysis demonstrated differences in the spatial localization of cPLA(2)-IV and sPLA(2)-IIA protein in upper and lower segment myometrium. cPLA(2)-IV was predominantly in vascular endothelial cells, while sPLA(2)-IIA was observed in vascular, endothelial and smooth muscle cells. In addition, sPLA(2)-IIA showed a distinct nuclear or perinuclear localization in myometrial smooth muscle cells of the lower segment. We postulate that the increased expression of sPLA(2)-IIA rather than cPLA(2)-IV in the myometrium may play a role in the onset and/or maintenance of human parturition.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > RG Gynecology and obstetrics |
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Journal or Publication Title: | MOLECULAR HUMAN REPRODUCTION | ||||
Publisher: | OXFORD UNIV PRESS | ||||
ISSN: | 1360-9947 | ||||
Official Date: | 1 November 2004 | ||||
Dates: |
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Volume: | 10 | ||||
Number: | 11 | ||||
Number of Pages: | 7 | ||||
Page Range: | pp. 799-805 | ||||
DOI: | 10.1093/molehr/gah103 | ||||
Publication Status: | Published |
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