UNSPECIFIED. (2004) Labour is associated with increased expression of type-IIA secretory phospholipase A(2) but not type-IV cytosolic phospholipase A(2) in human myometrium. MOLECULAR HUMAN REPRODUCTION, 10 (11). pp. 799-805. ISSN 1360-9947

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Abstract

Human labour is associated with increased prostaglandin synthesis within the uterus. The aim of this study was to examine the expression of the type-IV cytosolic phospholipase A(2) (cPLA(2)-IV) and the type IIA secretory phospholipase A(2) (sPLA(2)-IIA) in myometrium in association with labour onset at term and preterm deliveries. These enzymes are important for the release of the prostaglandin precursor, arachidonic acid, from phospholipid membrane stores. RT-PCR was used to determine differences in gene expression between non-labour and labour groups. Expression of sPLA(2)-IIA in human myometrium was significantly increased with pregnancy, and with labour, both at term and preterm. Expression of cPLA(2)-IV in myometrium was not significantly altered with respect to pregnancy or labour. Immunohistochemical analysis demonstrated differences in the spatial localization of cPLA(2)-IV and sPLA(2)-IIA protein in upper and lower segment myometrium. cPLA(2)-IV was predominantly in vascular endothelial cells, while sPLA(2)-IIA was observed in vascular, endothelial and smooth muscle cells. In addition, sPLA(2)-IIA showed a distinct nuclear or perinuclear localization in myometrial smooth muscle cells of the lower segment. We postulate that the increased expression of sPLA(2)-IIA rather than cPLA(2)-IV in the myometrium may play a role in the onset and/or maintenance of human parturition.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology R Medicine > RG Gynecology and obstetrics
Journal or Publication Title:	MOLECULAR HUMAN REPRODUCTION
Publisher:	OXFORD UNIV PRESS
ISSN:	1360-9947
Date:	1 November 2004
Volume:	10
Number:	11
Number of Pages:	7
Page Range:	pp. 799-805
Identification Number:	10.1093/molehr/gah103
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/7849

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