The Library
Chemical probes for the functionalization of polyketide intermediates
Tools
Tools
Tools
Riva, Elena, Wilkening, Ina, Gazzola, Silvia, Li, W. M. Ariel, Smith, Luke, Leadlay, P. F. and Tosin, Manuela (2014) Chemical probes for the functionalization of polyketide intermediates. Angewandte Chemie International Edition, 53 (44). pp. 11944-11949. doi:10.1002/anie.201407448
|
PDF
WRAP_Riva_et_al-2014-Angewandte_Chemie_International_Edition.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (932Kb) | Preview |
Official URL: http://dx.doi.org/10.1002/anie.201407448
Abstract
A library of functionalized chemical probes capable of reacting with ketosynthase-bound biosynthetic intermediates was prepared and utilized to explore in vivo polyketide diversification. Fermentation of ACP mutants of S. lasaliensis in the presence of the probes generated a range of unnatural polyketide derivatives, including novel putative lasalocid A derivatives characterized by variable aryl ketone moieties and linear polyketide chains (bearing alkyne/azide handles and fluorine) flanking the polyether scaffold. By providing direct information on microorganism tolerance and enzyme processing of unnatural malonyl-ACP analogues, as well as on the amenability of unnatural polyketides to further structural modifications, the chemical probes constitute invaluable tools for the development of novel mutasynthesis and synthetic biology.
| Item Type: | Journal Article | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QP Physiology | ||||||||||
| Divisions: | Faculty of Science > Chemistry | ||||||||||
| Library of Congress Subject Headings (LCSH): | Molecular probes, Polyketides | ||||||||||
| Journal or Publication Title: | Angewandte Chemie International Edition | ||||||||||
| Publisher: | Wiley | ||||||||||
| ISSN: | 1433-7851 | ||||||||||
| Official Date: | 21 October 2014 | ||||||||||
| Dates: |
|
||||||||||
| Volume: | 53 | ||||||||||
| Number: | 44 | ||||||||||
| Number of Pages: | 6 | ||||||||||
| Page Range: | pp. 11944-11949 | ||||||||||
| DOI: | 10.1002/anie.201407448 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Open Access | ||||||||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), University of Warwick. Institute of Advanced Study (IAS), Marie Curie Intra-European Fellowship (IEF) | ||||||||||
| Grant number: | BB/J007250/1 (BBSRC) | ||||||||||
| Adapted As: |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
