UNSPECIFIED. (2004) Isolation and characterisation of bacterial strains containing enantioselective DMSO reductase activity: application to the kinetic resolution of racemic sulfoxides. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 65 (6). pp. 678-685. ISSN 0175-7598

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Abstract

The kinetic resolution of racemic sulfoxides by dimethyl sulfoxide (DMSO) reductases was investigated with a range of microorganisms. Three bacterial isolates (provisionally identified as Citrobacter braakii, Klebsiella sp. and Serratia sp.) expressing DMSO reductase activity were isolated from environmental samples by anaerobic enrichment with DMSO as terminal electron acceptor. The organisms reduced a diverse range of racemic sulfoxides to yield either residual enantiomer depending upon the strain used. C. braakii DMSO-11 exhibited wide substrate specificity that included dialkyl, diaryl and alkylaryl sulfoxides, and was unique in its ability to reduce the thiosulfinate 1,4-dihydrobenzo-2, 3-dithian-2-oxide. DMSO reductase was purified from the periplasmic fraction of C. braakii DMSO-11 and was used to demonstrate unequivocally that the DMSO reductase was responsible for enantiospecific reductive resolution of racemic sulfoxides.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology
Journal or Publication Title:	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Publisher:	SPRINGER
ISSN:	0175-7598
Date:	November 2004
Volume:	65
Number:	6
Number of Pages:	8
Page Range:	pp. 678-685
Identification Number:	10.1007/s00253-004-1667-6
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/7871

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