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Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation
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Zhu, Xinyao, Zhang, Nan, Wang, Zuobin and Liu, Xianping (2016) Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nanoindentation. Journal of Micro-Bio Robotics . doi:10.1007/s12213-016-0089-8 ISSN 2194-6418.
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WRAP_Zhu_9976741-es-270416-jmbr-d-15-00008_r1_.pdf - Accepted Version - Requires a PDF viewer. Download (2174Kb) | Preview |
Official URL: http://dx.doi.org/10.1007/s12213-016-0089-8
Abstract
In this study, we presented an investigation of mechanical properties by AFM nanoindentation on human hepatocellular carcinoma cells treated with fullerenol for 24, 48 and 72 hours. AFM nanoindentation was routinely applied to investigate the morphology and biomechanical properties of living carcinoma cells, and adhesion phenomena (negative force) were detected in the obtained force-displacement curves. Conventionally, Hertz contact model has been widely used for determination of cell elasticity, however this contact model cannot account for adhesion. Alternatively, JKR contact model, as expected for adhesion circumstance, has been applied to fit the obtained force-displacement curves. In this investigation, we have derived both the work of adhesion and the elastic modulus of biological cells (human hepatocellular carcinoma) under fullerenol treatment. The results show that the chosen JKR model can provide better fitting results than Hertz contact model. The results show that both Young’s modulus and work of adhesion exhibit significant variation as the treatment time increases. The calculated mechanical properties of elastic modulus and work of adhesion can be used as an effective bio-index to evaluate the effects of fullerenol or other anticancer agents on cancer cells and thus to provide insight into cancer progression in the treatment.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > Engineering | ||||||||
Journal or Publication Title: | Journal of Micro-Bio Robotics | ||||||||
Publisher: | Springer | ||||||||
ISSN: | 2194-6418 | ||||||||
Official Date: | 7 May 2016 | ||||||||
Dates: |
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DOI: | 10.1007/s12213-016-0089-8 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||
Date of first compliant deposit: | 29 April 2016 | ||||||||
Date of first compliant Open Access: | 7 May 2017 |
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